In the upper Amazon River , Brazil, it was observed in the large family farm variability pumpkin landraces, which is widely used in food. Local cultivars are grown in small areas, for consumption in agricultural production and marketing unit in regional markets. The varieties of family agriculture are important sources of variability of germplasm characterization and require for their best knowledge. They still represent a featured action for the knowledge of genetic variability in conservation programs, use and breeding of the species.The Cucurbita descriptors list elaborates on vegetation data  and reproductivity, environmental development characteristics,health, origin, geographic distribution, domestication, uses and importance of food and nutrition,hydroponic dutch buckets conservation, breeding, among others .The aim of this study was to estimate the current level of genetic variability of local cultivars by means of estimation techniques of genetic parameters.

The areas of collection of local cultivars samples were family agriculture production units located in high floodplain ecosystems and low characterized by wetlands with white water , in the Benjamin Constant, Upper Solimoes River and Iranduba, the Lower Solimoes River, Amazon, Brazil .In sampling there were adopted the procedures for out crossing species, such as the case of pumpkin land races, to collect material in field conditions with gametic control. In general, farmers adopt the system of pumpkin perform cultivation for simultaneous production of fruit for consumption, sale and to obtain seeds for the next cropcycle, crop lands were stratified.The experimental test was conducted at the Horticultural Experimental Station, located at Agricultural Park State Government in the Afi climate Koppen . The soil is classified as Red-Yellow Argisol Alic, sandy texture and low natural fertility. The experimental area is located on land with slope in the east-west direction and is divided into six terraces distributed in south-north direction. On the terraces located in the upper part of the thickness of the surface layer of sandy soil is thinner compared to the terraces located in the lower part.

The test took the first three terraces of the Experimental Station and each block held a terrace where were evaluated six local cultivars and a witness and six families of half sib progeny . The subplot was composed of six plants, a spacing of 3.0 × 3.0 m  evaluated in three repetitions , totaling 756 plants in the experiment. From the field test it was possible to estimate the variability between and within local varieties and families of half sib.For the experiment was adopted the randomized block design with split plots where treatments were local cultivars sampled in five areas of family farming in the state and a commercial cultivar bato bucket. The results of chemical analysis showed that the three areas of soil collection showed organic matter carbon content considered good and an acidity ranging from low to more low. With respect to particle size, the results indicated sandy soil in Block 3, located on the terrace 3.For the soil analysis data of particle size distribution and the degree of compression given to the sand content was higher in block three of the assay. Given this fact, some factors must be considered to understand the performance and development of local cultivars and witness.

The major composition of the sand ground is strong contributor to higher water stress, low humidity for retention in macropores greater loss of nutrients and increased heating of the root system.Moreover, observations obtained from experiments with vegetable showed that these differences in thickness of the surface sandy soil layer between terraces may, in addition to causing differences in the availability of water and nutrients to plants, may interfere with higher or lower incidence of disease.The occurrence of disease may also be related to the loss of plant health. Plants with yellow leaf for environmental stress are susceptible to pathogen attack.The records were collected for the period from 6/6/2013 to 16/9/2013, for the transplanting period for final location and stage of harvest of ripe fruit. The rains were more frequent in the range of 27 June to 11 July and the end of last month until the first half of August. The rainfall increased again in the first half of September. The mean rainfall for the period was 3.6 mm/day.