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The far side is occupied by community and government facilities

Most of the search results involved tourist agencies in Nanjing and Shanghai who were advertising the theme park by means of a detailed tourist itinerary. Second, I found a limited number of advertisements for the sale of resettlement houses in the Jinhu New Village. Third, I found accounts from urbanites who had traveled to Jinhu and were commenting on their experiences at the theme park. Most of them seem to have enjoyed themselves and left only very short comments. Last but not least, I encountered one informed and detailed online report by a local resident—entitled “Jinhu New Countryside is a Gambling Game and a Fraud”. Although I have used the internet as an extra source to understand what is going on at the Jinhu site, it is worth noting that the internet has also served as an important arena used by actors involved with Jinhu to gain legitimacy, to voice complaints, to vent anger, and to advertise for economic profit. Jinhu New Village is located in southern Anhui Province about 30 kilometers south of Wuhu City. Altogether it occupies an area of 189 hectares , on a stretch of land running along the east side of the national road G205. G205 is a two-lane paved road built before China’s highway construction boom of the last ten years. The site is situated on a broad plain traversed by numerous canals and lakes. These lakes are the reason why this area would also be chosen as the site for a theme park. Given the relatively high average temperatures and annual precipitation, two harvests are possible each year . The rich agricultural fields in the Jinhu new countryside construction zone once supported twelve traditional villages . Residents of these villages have now all been relocated to Jinhu New Village on the southwest corner of the development zone. Both the Jinhu New Village and the associated theme park are separated from the national G205 road by a stretch of landscaping composed of a long canal, green lawns, and willow trees. As with many New Countryside projects now under construction in China—projects often readily visible to drivers rushing along the gleaming new freeways crisscrossing the country—the Jinhu site is a veritable monument to a particular notion of rural modernization and development.

When driving north along the national highway,maceta 5 litros one encounters first a large billboard explaining that one is approaching China’s “first low-carbon national tourist site.” Behind the billboard, one sees the new houses arranged in a perfect grid, quite distinct from the more haphazard arrangement of farmhouses and fields in the traditional villages . Less than a kilometer beyond the New Village, one reaches the entrance to Jinhu Rural World theme park, situated on the east side of the highway. Here two large billboards announce: “National AAAA tourist site—National modern technology agricultural experimental site—National agricultural tourist demonstration site—Welcome to Jinhu experimental site!” According to the master plan—which appears on one of the billboards— the rural world theme park will eventually have 41 attractions. However, as of June 2012, only 16 projects were finished and open to tourists. Below I analyze the built environment of Jinhu New Village and Jinhu Rural World theme park, paying particular attention to how rural space is undergoing transformation and to how the “New Countryside” contrasts with the old countryside and traditional rural society.One’s first impression of Jinhu New Village is of a modern suburb imported from somewhere in the United States. It is a large housing complex, with houses that are remarkably uniform in structure and physical appearance laid out on a grid consisting of alleyways running either perpendicular or parallel to the national highway. The residences nearest to the highway are two- or three-story townhouses; further from the highway are several rows of new apartment buildings. The aesthetics of the new residences stand in stark contrast to the vernacular architectural style of the old red brick peasant houses, a few of which still stood undemolished just southeast of the New Village. This aesthetic of the New Village is a curious mimicry of American “streetcar” and “sitcom” suburbs, those suburbs that Dolores Hayden describes as refilecting the American “idealized life in single-family houses with generous yards”. More specifically, the dwellings in the New Village, with their white stucco walls, their grey-tile roofs, and their uniformity of appearance, resemble in remarkable ways tract housing in American suburbia—such as in the Los Angeles Basin . As in any American suburb, each house also has a garage, with additional parking available in clearly marked parking spaces along the alleyways. Many aspects of this new Chinese suburbia seem out of place in the Chinese context.

Grass lawns may have particular symbolic significance in American urban and suburban environments , but from a Chinese peasant’s perspective, grass is a weed that infects one’s fields, the last thing one would think of deliberately planting beside one’s home. The garages and parking lots are also curiosities given that very few peasant households in Jinhu own a car. Many peasants currently use their garages to store their agricultural tools. The New Village does not merely emulate American suburbia; it also contains elements of a China-specific vision of the urban modern, as represented by an orderly but dense arrangement of tall buildings. In American suburbia, the ideal consists of single detached family houses placed in a landscaping that tries to imitate the natural environment. However, in Jinhu New Village, one experiences a much greater feeling of density, highlighted by the concentration of residences, including both rows of two- to three-story stucco houses—called bieshu in Chinese—and blocks of six- to seven-story apartment buildings. The term bieshu ordinarily refers to detached houses. At Jinhu, however, they are not free-standing; they are much more like townhouses or row houses than detached single-family houses. One way to explain this preference for a densely built environment is to consider the perceived ecological limitations of Chinese agricultural land. The central government has in recent years expressed concern for China’s “national food security,” leading to various strategies to maximize available arable land, a point I will address later in detail. However, there is another equally plausible explanation. For the Chinese urban elites, tall buildings and uniform orderliness symbolize modern life. For example, many Chinese government officials and nouveaux riches traveling to the U.S. are quite disappointed by their experience—if the U.S. is so modern, why do the vast dense expanses of awe-inspiring skyscrapers exist only in New York City and Chicago, and not in other American cities and towns?10 The orderliness and density of Jinhu New Village, then, is a public advertisement of how fortunate the displaced peasants are to be living in a new modern environment. Besides the modern architectural styles and the orderly arrangement of buildings,cultivo de la frambuesa the modern environment of Jinhu New Village is also refilected inside the homes. Here, one finds a variety of conveniences unavailable in the old villages, including flush toilets, solar powered water heaters, running water, reliable plumbing, built-in gas stoves, garbage collection, and even high-speed internet.

Although my host Mrs. Tang had only a second grade education, she was adamant about the importance of using computers and accessing the internet, by means of which she learned a great deal chatting with other netizens. Indeed, computers and the internet seem generally to be welcome by most peasants in rural China. Other particularly appreciated modern conveniences are showers and hot water, made possible by the solar-powered water heaters installed on the roofs of the houses. I have observed these new water heaters in New Countryside housing projects . Much like the entire Jinhu development site, the space of the New Village is clearly compartmentalized with well-delineated commercial, communal, governmental, and private residential zones. The core of the New Village is organized around a T-shaped axis . Immediately after entering the New Village by the main gate, one faces a two-lane street with commercial shops on both sides . At the far end of this street is a perpendicular street that forms the top of the T. One side of this second street is also occupied by commercial space. A multi-story building houses the police station, the community administrative office , and a “petition office” that handles grievances.Beside the government building is a three-story pre-school and kindergarten, a basketball court, and an indoor market place . Further from the T-shaped axis and on all sides are the private residences. The two- or three-story townhouses stand closer to the national highway; the apartment blocks lie just beyond the community and administration buildings. Throughout the New Village, iron fences separate public spaces from private residential yards, and curbs demarcate the boundaries between pedestrian and vehicular zones of circulation. None of these various ways of compartmentalizing space—derived from Western urban models—were present in the old natural villages. Despite the orderliness and the conveniences of the modern built environment, all is not as it seems, since the new houses come with a variety of extra costs that peasants were not burdened with in their old villages.

The cost of high-speed internet access is roughly USD $25 per month, a fee that also includes unlimited local phone calls. Peasants accept this fee more readily than some of the other charges they face, as it provides a service they did not formerly enjoy. By contrast, from the peasant’s perspective, the cost of water and natural gas are more difficult to accept. In the old villages, water was freely drawn from wells; and they used gathered firewood rather than gas for cooking. Most significant of all, however, their cost of living is now much higher because food has to be purchased instead of being produced on their land, a concept utterly alien to the peasants. While staying in the village, the most common complaint I heard was that residents no longer had land for cultivating vegetables and raising domestic animals. Now they had to buy food from supermarkets. Inevitably, village residents have found ways to cut living expenditures by a variety of means. To avoid using running water, women wash vegetables and laundry in the canal built next to the main road. For drinking water, many households invest in a well, which they dig in their small backyards. Not only is well water free, peasants also consider it to be cleaner than tap water. In a similar fashion, to minimize natural gas usage, many households have purchased portable pre-made cylinder-shaped aluminum stoves. Around 5 am every morning, in order to boil water, Mrs. Tang’s father-in-law got up to start a fire in the aluminum stove, using wood collected from demolished houses. This was a common practice: with the stove set right outside the garage door, he would chat—sometimes standing and sometimes sitting on a stool—with two other neighbors who were also doing the same thing. Another way to save on cooking expense is to build an old-style firewood stove in one’s backyard. One couple running a majiang parlor out of their home did just this, despite warnings from the New Village management, who has ordered them to demolish it, on the grounds that it was ugly and damaged the orderly and neat image of the New Village. There are other ways in which, in order to save money, residents resist the management’s efforts to maintain a neat appearance. To mitigate the daily cost of food, residents in Jinhu New Village utilize all sorts of marginal land around the edge of the complex. In some cases, they have gotten rid of the lawn near their houses by spreading herbicides left over from their farming days. Another less destructive way is to dig a hole just large enough to plant pumpkins or other vegetables that grow vertically, plants that can more discretely blend into the lawn and tree landscaping. Chickens are also allowed to roam freely on and around the landscaping . All of these various survival strategies constitute continuities with common practices in the old villages; all are very much part of the peasants’ familiar habitus.But the “landless” peasants residing in the New Village have also turned to other more novel strategies to help them make ends meet. Numerous residents have converted their townhouses for commercial purposes, something they would not have done in their old villages. One common small business is a restaurant.

Of the fruits evaluated the fewest isolates were obtained from grapes

Given that both methods capture genetic relatedness among accessions, a significant relationship between these two methods is expected.Principal component analysis revealed significant population structure defined by geography and use, but strong signals of genetic structure also exist at the level of pedigree relatedness. Previous work determined that 75% of the accessions evaluated here are related to at least one other accession by a first-degree relationship, and over half of the accessions are interrelated and form a single, complex pedigree network.Both the strong population-level and pedigree-level signals of genetic structure in our sample present challenges in genetic mapping as these are significant confounding factors when performing GWAS. Moreover, the rapid LD decay previously described for this and other diverse populations of V. vinifera suggests that millions of SNPs are required for well-powered GWAS in grapes.Despite our relatively low marker density and the challenges presented by strong genetic structure, we performed GWAS for all 33 phenotypes. For most traits, we found no convincing GWAS signals . However, we reasoned that we may find SNPs associated with key traits that experienced strong selection during domestication and breeding because selection results in extended LD surrounding the targeted loci, thereby requiring a lower SNP density than that required to map-unselected traits. We hypothesized that, by combining association mapping with selective sweep mapping ,macetas con drenaje we may identify loci associated with traits targeted during grape domestication and breeding. A key transition in grapevine domestication was the switch from dioecy to hermaphroditism: all wild Vitis species, including the ancestor of V. vinifera, are dioecious, and nearly all V. vinifera are hermaphroditic.

Hermaphroditism was likely the first, and arguably the most important, transition from wild vines to cultivated grapes: it enables self-pollination and subsequent clonal propagation of elite cultivars without the need for pollinators.Dioecy is found at low frequency in our sample: only 50 of the 550 accessions with flower sex data were labeled as dioecious. Despite this low frequency, we identified SNPs significantly associated with flower sex on chromosome 2 . The most significantly associated SNP overlaps with the 1.5 Mb region repeatedly identified via linkage mapping.This SNP is also found within the fine-mapped 143 kb region believed to harbor the causal flower sex locus.We therefore demonstrate that, even with only 50 accessions carrying the ancestral dioecy phenotype, we successfully map the flower sex locus at relatively high resolution using GWAS relative to traditional linkage mapping approaches. A genome-wide Fst scan comparing dioecious to hermaphroditic accessions also revealed that the SNP most strongly associated with flower sex had the highest Fst value genomewide, consistent with the effect of selection for hermaphroditism at this locus . If grape domestication resulted in a rapid increase in the frequency of the hermaphroditism allele, one would expect extended haplotype homozygosity, and thus extremely high xpEHH values, in and around the flower sex locus. While none of the xpEHH values at the flower sex locus fall within the top 1% most extreme values genome-wide, we do observe a suggestive peak with xpEHH values within the top 2.6% of genome-wide xpEHH values . xpEHH values in the bottom 1% of the genome-wide distribution are found directly adjacent to the flower sex locus identified here. We have no explanation for why a potential signature of selection could exist for dioecy in such close proximity to the flower sex locus. There are SNPs with extreme xpEHH and Fst values, indicating potential selection for hermaphroditism, at the distal end of chromosome 1 ranging from positions 366 to 467 kb . This genomic region overlaps with the region previously associated with flower sex in a bi-parental mapping population using the same Vitis9KSNP microarray employed for this study.

However, thisregion is several Mb from the locus highlighted in Figure 6a that color within a diffuse peak on chromosome 2 between 10 and has been repeatedly associated with flower sex. We hypothesize 17 Mb . Although the genomic region containing that this distal signal of selection is due to inaccurate localization significant GWAS hits for color overlaps the VvmybA1 gene, the of the array’s SNPs in the reference genome since, when this trait most significantly associated SNP found here is 3.6 Mb from the is mapped using genotyping-by-sequencing in the same bi- known causal mutation. Our inability to map the known color parental population, the flower sex colocalizes with the known locus with precision is consistent with results from rice and flower sex locus according to the reference genome.It is unclear Arabidopsis where markers with the strongest association why such mismapping occurs with the Vitis9KSNP array data, but signals were not found directly at known causative loci. Moreover, unexpected hybridization of non-targeted paralogous regions this result is unsurprising given the relatively low marker density may possibly contribute to these observations. of the SNP array employed here. Skin color in grapes is largely controlled by a single locus on While the diffuse association signal for grape color spanning chromosome 2, where a retrotransposon insertion in the MYBA1 nearly 7 Mb indicates that we have poor mapping resolution for gene results in a loss in pigmentation by disrupting anthocyanin this phenotype, it also suggests the presence of long-range LD biosynthesis.Although rare, white-skinned grapes have been potentially caused by selection. Saccharomyces cerevisiae is an important experimental model organism in addition to its commercial significance as the predominant yeast species during wine fermentation. Modern strains of S. cerevisiae are thought to have arisen in Asia given the diversity of strains and reproductive isolation observed in a study of S. cerevisiae isolates from human-associated and non-human-associated environments in China .

Distinct linages were observed for isolates from primeval and secondary forests . However this study considered few isolates from wine environments and found fewer isolates of S. cerevisiae from fruit sources and more from tree bark, rotting wood and soil samples than from fruit samples. The authors concluded that grape and orchard isolates were similar to those of the wine European lineage. Our goal was to evaluate in greater depth the diversity of natural vineyard isolates from two wine regions in China. Several studies have reported on the genetic diversity of S. cerevisiae strains in different wine-producing regions. These studies revealed that geographic region , climatic conditions , vintage , grape varieties and must characteristics , inoculation of starter yeasts , and SO2 addition affected the diversity of S. cerevisiae observed. In many cases genetically distinct strains of S. cerevisiae were isolated from the same fermentation during wine fermentation . The diversity of S. cerevisiae strains present in fermentations has been shown to play an important role in the characteristics of the final product . Numerous molecular methods have been developed to study the ecology and population dynamics of S. cerevisiae strains . Interdelta sequencing typing uses the variation of the number and position of the delta element, a repeated sequence that flanks the Ty1/Ty2 retrotransposon , that allows interpreting strain similarities and evolutionary or adaptive distance . A succession of different S. cerevisiae strains are established during native as well as inoculated fermentations that could have positive or negative effects on the course of fermentation and wine quality . Vezinhet et al. analyzed the evolution of S. cerevisiae strains isolated from spontaneous fermentations during six consecutive years. These authors concluded that the wide distribution of some strains in the studied areas and their presence over years,macetas 7 litros constitute evidence for the occurrence of specific indigenous strains representative of an enological region. China is an important wine-producing country and while some studies have investigated indigenous yeast species and population dynamics during wine fermentation within local viticulture regions ; few studies have focused on the breadth of the diversity of S. cerevisiae wine genetic resources of China. A study of human- and non-human-associated strains of China found novel distinct lineages only distantly related to the wine strain linages . The genetic diversity and relatedness of indigenous wine S. cerevisiae resources have not been extensively compared with that of wine strains isolated from other geographic regions. Ningxia and Xinjiang provinces, where the strains in this study were isolated, are two of the oldest wine producing regions in China. Shanshan, Xinjiang in northwestern China belongs to a temperate continental climate, with an average temperature of 12˚C. It is situated 92°22′E, 42°87′N with an average altitude of 3986 m.

Qing Tongxia, Ningxia in north central China also belongs to a temperate zone with an arid and semi-arid climate. It is situated 105°21′ to 105°21′ E, 37°36′ to 38°15′N with an average altitude of 1118 m. A comparison of the genetic diversity of S. cerevisiae resources in different viticulture regions of China with isolates from other diverse geographical regions is of importance to the study of global S. cerevisiae ecology. In the present study, interdelta sequence typing with improved primers was used as genetic marker for the distinction of S. cerevisiae strains. Dendrograms were constructed based on similarity among different patterns of bands and the genetic relationships of all strains were evaluated. The strains used in the study were either isolated from fermentations of different grape varieties in the Ningxia and Xinjiang Provinces in China or obtained from the Department of Viticulture and Enology Culture Collection at the University of California, Davis. The aims of the present work were to evaluate the genetic diversity and relatedness amongSaccharomyces strains of different geographic origin, to establish a strain collection to preserve the S. cerevisiae genetic resources of China, and to identify strains useful for further development for commercial wine production in China. Fifty-four isolates collected from fifteen spontaneous fermentations of grapes grown in China and one commonly used commercial yeast, Lavin RC212, were used in this study and obtained from the collections of the College of Enology, Northwest A&F University, Yangling, Shaanxi, China. This set of strains was selected on the basis of interdelta sequence profiles from a total of 349 isolates collected from fifteen spontaneous fermentations of grapes grown in Shanshan, Xinjiang and Qing Tongxia, Ningxia. Fifty-nine yeast colonies were isolated from six spontaneous fermentations of different commonly used grape varieties: Red Globe, Small-berry Thompson Seedless, Big-berry Thompson Seedless, Merlot, Mixed red and Mixed white in Xinjiang. Two hundred ninety isolates were obtained from nine spontaneous fermentations of the grape varieties Cabernet Gernischt, Cabernet Sauvignon, Cinsault, Merlot, Pinot Noir, Riesling, Sauvignon Blanc, Semillon, and Yan73 in Ningxia . The grape must fermentations were allowed to proceed spontaneously at 25~28˚C for 7~11 days until dry. Fermentations were sampled at early, mid and the final stage of fermentation, and serial ten-fold dilutions were inoculated onto WLN and incubated for five days at 28˚C.These yeasts were differentiated and classified according to colony morphology and color. S. cerevisiae isolates were purified and then maintained in 20% glycerol at -80 °C until further analysis, resulting in the selected set of 349 isolates for the interdelta sequence analysis. The composition of the different grape musts is reported in Supplemental Table 1 for Ningxia and in Supplemental Table 2 for the fermentations from Xinjiang. The fifty-four yeast strains selected from this larger population of isolates represented the major strain clusters of interdelta sequence profiles identified in the earlier preliminary study. The origins of the 54 isolates used in this study are shown in Table 1. Identification of S. cerevisiae was confirmed by PCR-RFLP of the 5.8S-ITS rDNA using restriction enzymes HaeIII, HpaII, and ScrFI as described by Li et al. . Strains were maintained in frozen stocks at -80˚C before use. Note that a similar strain numbering system was independently used by Wang et al. in their study but the strains are unrelated. We retained our numbering system since that is the designation given to the strains in the Northwest A&F University strain collection. Other strains were obtained from the Wine Yeast and Bacteria Collection of the Department of Viticulture and Enology at the University of California, Davis. The data from all fifty-two Saccharomyces isolates listed in Table 1 of Liu et al. were included in this study as the method of interdelta sequence analysis was identical. These yeast strains were collected from California, France, Italy, Northern Europe, and Spain .The interdelta sequence patterns obtained after gel electrophoresis were used for the construction of a presence/absence matrix, taking into account the total number of different bands observed. The interdelta sequence patterns were obtained following electrophoresis.