Category Archives: Agriculture

Original leaves were designated as leaves present on the seedling at the beginning of the experiment

High concentrations of Na+ in the cytoplasm disrupt the ionic balance and the uptake of essential mineral nutrients, such as K+, which in turn causes adverse effects on many metabolic pathways. To cope with salt stress, plants have evolved various tolerance mechanisms including two transport processes at the single cell level. Either exporting Na+ out of the cell, or compartmentalizing excessive Na+ into the vacuole. These two transport mechanisms act in a coordinated manner to maintain a low Na+ concentration in the cytoplasm. However, it remains unknown if they are regulated by the same or different signaling pathways. The SOS pathway is generally viewed as a signaling mechanism for the activation of the Na+ efflux through SOS1, a NHX-type Na+/H+ exchanger in the plasma membrane. The loss of function of SOS genes thus results in hypersensitivity to NaCl, coupled with the Na+ over-accumulation in the cytoplasm. On the other hand, some Na+/H+ exchangers are localized in the tonoplast and may be involved in transporting Na+ from the cytoplasm to the vacuole. However, the exact role of different NHX isoforms responsible for salt tolerance remains unclear. Interestingly, the two distinct but inter-connected salt transport processes appear to be both regulated by calcium signaling, in which calcineurin B-like proteins are thought to be the primary calcium sensors during salt stress adaptation. Among them, CBL4 and CBL10 display distinct tissue expression patterns and subcellular localizations. The spatial specificity of these two calcium sensors may contribute to their functional diversification in salt stress adaptation. In order to understand how they work synergistically in the regulation of salt tolerance,plastic pots 30 liters we genetically analyzed the salt-sensitive phenotype of the cbl4 cbl10 double mutant in comparison with the single mutants.

The cbl4 cbl10 double mutant was dramatically more sensitive to salt as compared to the cbl10 and cbl4 single mutants, suggesting that CBL4 and CBL10 either functionally overlap or each directs an independent salt-tolerance pathway. If the two CBLs are functionally overlapping, they should regulate the same transport processes and then the double mutant should not only show more severe phenotype but also show more severe deviation in the Na+ and K+ contents as compared to the wild-type plants. However, that was not the case: cbl4 and cbl10 displayed generally opposite Na+ and K+ profiles. Although the cbl4 cbl10 double mutant plants showed Na+ over-accumulation compared to the wild type, but significantly lower Na+ content than the cbl4 single mutant . This suggests that CBL10 should not be involved in the CBL4-regulated Na+ extrusion process , although these two calcium sensors interact with a common downstream kinase CIPK24 . Instead, CBL10 should regulate a distinct Na+-transport process in response to high salt, probably the Na+ sequestration into the vacuole, as suggested by its tonoplast localization and the lower Na+ content in the cbl10 mutants. This is consistent with the general theme that the Na+ efflux or Na+ sequestration into the vacuole both contribute to salt tolerance and disrupting either may result in elevation of the Na level in the cytoplasm and thus leading to salt sensitivity. Certainly disrupting both transport processes would lead to more severe salt sensitivity, which match the more sensitive phenotype of cbl4 cbl10. Previous studies suggested that CIPK24 serves as the common downstream target of CBL4 and CBL10 by forming CBL4-CIPK24 or CBL10-CIPK24 complex at the plasma or vacuolar membrane separately. Although our findings in this study supported this hypothesis, they also suggested that other CIPKs, in addition to CIPK24, should be also involved in the CBL10-mediated pathway based on the genetic evidence that double mutants of cbl4 cbl10 and cipk24 cbl10 displayed a significant enhancement in Na+ sensitivity as compared to cipk24 .

Indeed, screened by the yeast two-hybrid assay, we found that CBL10 did interact with other CIPKs in addition to CIPK24 . Various combinations of CBL10 with different CIPKs may target different target proteins and exhibit diverse functions. To examine whether SOS1 is a downstream component of CBL10 in the pathway, we also compared the salt sensitivity between sos1 cbl10 and sos1. In our test conditions, the salt sensitivity of cbl4 cbl10 and sos1 cbl10 was comparable to sos1 , suggesting that SOS1 may serve as aconverging point for the two CBL pathways. However, the double mutants cbl4 cbl10 and sos1 cbl10 accumulated much lower Na+ content than the single mutants of cbl4 and sos1, respectively, under salt conditions , which implies that CBL10 and SOS1 functions in two different transport processes in regulating Na+ homeostasis. For instance, in the sos single mutants in which the Na+ efflux is blocked, the CBL10 pathway functions to transport Na+ into the vacuole leading to the over-accumulation of Na+ in plant tissues. When the vacuole sequestration is defective in the cbl10-associated double mutants, the Na+ uptake is inhibited as a feedback of lacking storage space, leading to less accumulation and thus lower Na+ content in these double mutants as compared to the sos single mutants . Despite overall lower Na+ content in plant tissues, the double mutants showed similar salt sensitivity as sos1 because the majority of Na+ in these double mutants is in the cytoplasm effectively causing toxicity. Our results thus provide an example where a two-tier evaluation system must be implemented for dissecting salt tolerance mechanism in plants: First by whole-plant phenotyping and further by the analysis of Na+/K+ homeostasis . Concerning the target transporters for CBL10, all evidence so far supports the hypothesis that the CBL10-CIPK pathway may regulate Na-transporters in the tonoplast. Sequestration of Na+ into the vacuole is presumably fulfilled by an array of Na + transporters that include the vacuole-localized NHX-type Na+ /H+ transporters.

However, recent genetic evidence indicates that vacuole-localized antiporters NHX1-4 have Na+-transport activities but may not contribute much to the vacuolar Na+ compartmentation, because the quadruple knockout mutant nhx1/2/3/4 is not more sensitive to NaCl than the wild type. Furthermore, vacuoles isolated from the quadruple mutant still retain the Na+ uptake that is independent to the pH gradient, implicating the presence of NHX-independent Na+ transporters in Arabidopsis vacuoles. We speculate that some of these unknown transporters may serve as CBL10-CIPK targets. On the other hand, endosomal compartments emerge as critical players that may be directly involved in controlling Na+ homeostasis. A possible but yet to be proved model is that the Na+ sequestration into the plant vacuole may actually be achieved, at least in part,round plastic pots through endosomal Na+ scavenging processes and subsequent fusion to the vacuole. NHX5 and NHX6 are localized to endosomal compartments and associated with protein trafficking from the Golgi/Trans-Golgi Network to vacuoles. Supporting this hypothesis is the finding that disruption of two endosomal NHXs in the nhx5 nhx6 double mutant showed increased sensitivity to salinity . Considering the fact that a proportion of the CBL10 protein was also localized to the dynamic endosomal compartments, NHX5/6 could also act as the candidate targets of the CBL10-CIPK complexes. In a recent work, translocon of the outer membrane of the chloroplasts 34 was identified as a novel interaction partner protein of CBL10 at the outer membrane of chloroplasts, clearly indicating that CBL10 can relay Ca2+ signals in more diverse ways than currently known.Identification of target transporter directly regulated by the CBL10-CIPK module is an important and challenging task for future research, which would also unravel the pathway through which Na+ is deposited into the plant vacuole. Treated wastewater, commonly called reclaimed or recycled water, is a valuable water source in arid and semi-arid areas where fresh water sources are becoming increasingly scarce due to urbanization and climate change . Reclaimed water may have many beneficial applications, including agriculture irrigation and landscape irrigation. In the state of California, these irrigation uses account for 37% and 18%, respectively, of the 650,000 acre-feet per year of water reuse . State policy calls to increase the use of reclaimed water to more than 2.5 million acre-feet per year by 2030 . Accompanying increased reuse, the presence and environmental risks of unregulated organic contaminants in reclaimed water are drawing attention . Pharmaceutical and personal care products and endocrine disrupting compounds are typically anthropogenic chemicals with known biological effects that may interfere with normal metabolism and behaviors of organisms .

Many PPCP/EDCs are routinely found in reclaimed water , as well as in surface water impacted by wastewater treatment plant effluent and in groundwater . When reclaimed water is used for irrigation, the associated PPCP/ EDCs may interact with the soil matrix and may contaminate groundwater and food crops . Accumulation of PPCP/EDCs into food crops that are consumed fresh, such as many leafy vegetables, is relevant due to the likelihood of unintentional human exposure. If research demonstrates that accumulation of PPCP/EDCs by crops is unlikely to result in human health risks, this will provide scientific basis to promote use of reclaimed water, as well as enhance positive public perception of water reuse. Many factors influence the uptake of organic compounds into plants, such as by affecting diffusion through cell membranes. Briggs et al. suggested that chemical hydrophobicity is an important factor affecting uptake by diffusion and that chemicals with a log Kow of 1 – 3.5 have the greatest plant uptake potential because lipid and aqueous solubility are balanced . In addition to hydrophobicity, molecular ionization has also been shown to influence plant accumulation, such as of herbicides . Charged molecules may have a reduced potential for plant uptake, since ionization may reduce their ability to permeate cell membranes . However, the role of ionization is poorly understood and exceptions have been noted . To date only a handful of studies have considered plant uptake of PPCP/EDCs . While these studies have clearly shown the ability for plants to take up PPCP/EDCs, the state of knowledge is limited to a few compounds or plant types. Due to the analytical challenges of detecting chemicals at trace levels in plant matrices, most studies also relied on the use of artificially high concentrations, with a few exceptions . In this study, we comparatively determined the accumulation of four commonly occurring PPCP/EDCs, i.e., bisphenol A , diclofenac , naproxen , or nonylphenol , at relevant environmental levels into two leafy vegetables, lettuce and collards, and examined the composition and distribution of accumulated residues. These compounds have been frequently detected in reclaimed water and surface water , and have different ionization states at neutral pH. To achieve realistically low concentrations while affording quantitative measurement, 14C-labeled compounds were used. Results were used to infer effects of plant type and compound characteristics on plant accumulation and estimate probable human intakes. Following 21 d of hydroponic cultivation, plants were sacrificed for analysis of 14C accumulation and distribution. Each whole plant was rinsed with DI water, and then separated into roots, stems, new leaves, and original leaves.Individual plant samples were placed in pre-weighed metal screen pouches, weighed to determine wet weight, and dried at 50 °C for 60 h. After drying, each plant sample was weighed to measure the dry weight, and then chopped and mixed in a stainless steel coffee grinder. The grinder was rinsed between samples with DI water and methanol to prevent cross contamination. Multiple 150 mg sub-samples of each plant sample were analyzed until standard deviation of the sub-samples was below 20%, due to notable variation in plant tissue activity. Sub-samples were combusted on an OX-500 Biological Oxidizer at 900 °C for 4 min, and the evolved 14CO2 was trapped in 15 mL of Harvey Carbon-14 cocktail . The 14C was measured on a Beckman LS 5000TD Liquid Scintillation Counter . Recovery was 91-96% for spiked standards,which was used to correct for the actual activity. The activity and weight of the sub-samples were used to determine the total radioactivity accumulated in different tissues of each plant. Analysis of 14C by combustion provided information on total residue in plant tissues. To better understand the nature of the residue, plant samples were solvent extracted using a method modified from Wu et al. . The fractions of 14C in solvent-extractable and nonextractable forms were separately determined.

Cuttings were placed in pots with approximately 400 cm3 of potting soil in a greenhouse

One and two weeks after inoculation, the roots were carefully removed from the Magenta jars, were rinsed, and were prepared either for GUS-staining or for viewing under a Zeiss Axiophot fluorescent microscope. Nodulation in potting soil. Stem cuttings of the transgenic alfalfa plants were made as described above and allowed to root.One week before inoculation, nitrogen nutrition was withdrawn from the plants, but other macronutrients were supplied. The potting soil was leached with large quantities of tap water four and one days before inoculation. Rm1021 cells were grown in RDM medium , containing 100 mg of streptomycin per liter to an OD600 of 0.11 or 0.13, depending on the experiment. Rhizobia were pelleted in a clinical centrifuge and were suspended in sterile milli-Q water to an OD600 of 0.1 . Rm1021 suspension was placed on the surface of the potting soil of each plant. The plants were grown for 21 dpi. Stems were cut off at the crown, and the potting soil was gently removed from the nodulated roots in standing tap water. The nodules were separated from the roots, were divided into pink and senescent types, and were counted. The external morphology of the nodules was also examined. Nodulation in Turface rooting medium. Rooted cuttings were placed in pots with approximately 400 cm3 of inert Turface rooting medium and were allowed to grow in the presence of a complete, dilute nutrient solution. One day before inoculation, nitrogen was withdrawn from the plants. Rm1021, grown to early stationary phase,barley fodder system was prepared as described above. Rm1021 suspension was inoculated onto each plant. The plants grew for 34 more days, and then, the stems were cut off at the crown. The Turface was removed from the nodulated roots in standing tap water. The external nodule morphology was examined. Nodulation under hydroponic conditions. Stem cuttings were allowed to root as described above.

Fluorescent light grates were covered with aluminum foil, and individual square openings of the grate, five to six squares apart, were cut out for placement of plants. The rooting medium was gently removed from the roots of the cuttings by placing them in standing tap water. The crown of each rooted cutting was wrapped in cotton and firmly wedged into an opening of a fluorescent light grate. Rooted cuttings were spaced evenly, with 30 cuttings per grate. Each grate was placed on top of a tank containing 30 liters of complete 1 /4-strength Hoagland’s medium. Tanks were continuously aerated with aquarium pumps. Six independent vector control, 12 independent LEC1AS, and 12 independent LEC2AS plant lines were used in each of six hydroponic tanks. The entire assembly of 30 plants could be removed and replaced relatively undisturbed from the medium. The complete nutrient solution was replaced with 30 liters of ¼-strength Hoagland’s medium lacking nitrogen. Five days after medium replacement, a suspension of Rm1021, prepared as described above, was uniformly mixed into the medium, and the roots were returned to the solution. Rm1021 inocula from mid-lag, early-exponential, late-exponential, early-stationary, or stationary phase were used. The liquid level of the hydroponic tanks was maintained by adding deionized water. The plants grew for an additional 28 to 37 dpi. Stems of nodulated plants were cut at the crown, were dried under vacuum at 45°C for 2 days, and were weighed. The nodulated roots were pooled for each plant type and were stored at –20°C; the nodules were later separated from the roots. Nodules and roots were dried under vacuum for 2 days at 45°C and were weighed. Some nodulated roots were left intact, were allowed to dry at room temperature under ambient conditions for 14 days, and then were weighed. Uninoculated plants were removed from the hydroponic conditions, were dried in the greenhouse for 1 week, and then, the roots and vegetative tissues were separated at the crowns and weighed. With the growing population and limited freshwater resources, there is increased interest in water conservation practices like using recycled wastewater and hydroponic agriculture. The presence of pathogens in the associated environmental compartments exposes a large fraction of the general populace to infection risks. Therefore, a need of the hour is ensuring that our infrastructure meets the safety requirements designed to protect human health.

Proper disposal and treatment of wastes generated at hospitals, industries and residences help meet this goal by reducing the pathogen loads in the environment. However, complete elimination of pathogens is not an option. Therefore, a framework to quantify the threat to human health is desired. The popularly adopted framework is called Quantitative Microbial Risk Assessment or QMRA. With the growing population and limited freshwater resources, there is increased interest in water conservation practices like using recycled wastewater and hydroponic agriculture. The presence of pathogens in the associated environmental compartments exposes a significant fraction of the general populace to infection risks. Therefore, a need of the hour is ensuring that our infrastructure meets the safety requirements designed to protect human health. Proper disposal and treatment of wastes generated at hospitals, industries, and residences help achieve this goal by reducing the pathogen loads in the environment. However, complete elimination of pathogens is not an option. Therefore, a framework to quantify the threat to human health is desired. The popularly adopted framework is called Quantitative Microbial Risk Assessment or QMRA. The main tenets of QMRA are as follows: 1) hazard identification; 2) exposure assessment; 3) dose-response modeling; 4) risk characterization, and 5) risk management. Hazard identification constitutes deciding on the system of interest and listing out the pathogens present/expected in that system. After identifying the hazard, the interaction of people with the system are modeled to quantify exposure to the pathogen. Suppose the system of interest is a particular lake used for recreation, and the hazard identified is E. coli. Exposure assessment would entail enumerating the E. coli finally ingested by the person . These processes have a lot of associated variability and uncertainty. Therefore, quantities are stratified by groups or represented by distributions rather than point estimates. Estimating the risk while accounting for these variabilities and uncertainties is done by Monte Carlo sampling. Dose-response models relate the number of the pathogen to the probability of a person falling ill . They are constructed with data from clinical trials in which a predetermined dose of pathogens is administered to a cohort of subjects and the number falling ill counted. The latter is then divided by the total number of subjects to reflect the probability of a single person falling ill.

This process is repeated for different pathogen doses to generate data for the models. While these clinical trials may use animals, datasets generated from human trials are preferred since they better reflect the human situation. Popular DRMs are the exponential and beta-Poisson models. DRMs for different pathogens may share the same functional form but differ in the numerical values of model parameters as a consequence of the biological differences between the pathogens. Risk characterization involves calculating the risk posed by the hazard by integrating the output of the exposure assessment with the DRM of choice for that pathogen. One then compares these estimates with guidelines established by the U.S. Environmental Protection Agency or the World Health Organization . Based on these comparisons, risk management measures can be investigated in an iterative process by computing the risk posed by the intervention measures. Understanding the risk posed by ARB has been stymied by the absence of DRMs parameterized for ARB. This difficulty arises from the clinical trials used to parameterize current DRMs, which were performed using antibiotic sensitive bacteria . While we have invitro kinetic information relating ARB to ASB, the biophysical/kinetic interpretation of the parameters of the popular exponential and beta-Poisson DRMs is not straightforward. Moreover,hydroponic barley fodder system the dose-response outcome is potentially complicated by the other processes at play, such as horizontal gene transfer and the differential influence of antibiotics on ASB and ARB death rates. The resulting illness may or may not respond to antibiotic treatment if the ARB sub-population persists. These challenges require a mathematical framework capable of handling the underlying processes, which can then be used to perform risk assessments of ARB and determine the best course of action. A point of longstanding debate in QMRA, and broadly the topic of disease progression, is the hypothesis of independent action. It proposes that pathogens act independently of one another, and each has a probability p of initiating infection. The alternative hypothesis is one of cooperation where infection is expected when more than one organism survives to overwhelm the host’s defenses collectively. DRMs assuming independent action have wider acceptance than DRMs which assume cooperativity. However, DRMs with cooperativity consider the cumulative effects of bacteria but not the potential synergistic interactions between bacterial cells or quorum sensing. I believe that incorporating cell-cell interaction in dose-response is an essential step to developing a better understanding of the development of disease and its treatment.Risk estimates for lettuce grown in the hydroponic tank or soil are presented in Fig. 2.4. Across these systems, the FP model predicted the highest risk while the 1F1 model predicted the lowest risk.

For a given risk model, higher risk was predicted in the hydroponic system than in the soil. This is a consequence of the very low detachment rates in soil compared to the attachment rates. Comparison of results from Sc1 and Sc2 of soil grown lettuce indicated lower risks and disease burdens under Sc1 . Comparing with the safety guidelines, the lowest risk predicted in the hydroponic system is higher than the U.S. EPA defined acceptable annual drinking water risk of 104 for each risk model. The annual burdens are also above the 106 benchmark recommended by the WHO. In the case of soil grown lettuce, neither Sc1 nor Sc2 met the U.S. EPA safety benchmark. Two risk models predicted borderline disease burden according to the WHO benchmark, for soil grown lettuce in Sc1, but under Sc2 the risk still did not meet the safety guideline. I found that neither increasing holding time of the lettuce to two days after harvesting nor using bigger tanks significantly altered the predicted risk . In comparison, the risk estimates of are higher than range of soil grown lettuce outcomes presented here for 2 of 3 models. The SCSA sensitivity indices are presented in Fig. 2.5. For hydroponically grown lettuce, the top 3 factors influencing daily risk are amount of lettuce consumed, time since last irrigation and the term involving consumption and ρshoot. Also, the risk estimates are robust to the fitted parameters despite low identifiability of some model parameters . For soil grown lettuce, kp appears to be the major influential parameter, followed by the input viral concentration in irrigation water and the lettuce harvest time. Scorr is near zero, suggesting lesser influence of correlation in the input parameters. To predict viral transport in plant tissue, it is necessary to couple mathematical assumptions with an understanding of the underlying biogeochemical processes governing virus removal, plant growth, growth conditions and virus-plant interactions. For example, although a simple transport model without AD could predict the viral load in the lettuce at harvest, it failed to capture the initial curvature in the viral load in the growth medium . An alternative to the AD hypothesis that could capture this curvature is the existence of two populations of viruses as used in, one decaying slower than the other. However, I did not adopt this approach as the double exponential model is not time invariant. This means that the time taken to decay from a concentration C1 to C2 is not unique and depends on the history of the events that occurred . Other viral models, such as the ones used in faced the same issues. Incorporating AD made the model time invariant and always provided the same time for decay between two given concentrations. This model fitting experience showcases how mathematics can guide the understanding of biological mechanisms. The hypothesis of two different NoV populations is less plausible than that of viral attachment and detachment to the hydroponic tank. While it appears that incorporating the AD mechanism does not significantly improve the accuracy of viral load predictions in the lettuce shoot at harvest, this is a consequence of force fitting the model to data under the given conditions.

Potential agricultural losses are exacerbated by a history of pesticide resistance development

Many antibiotics and other common contaminants of emerging concern can be excreted by both humans and animals with little change in their chemical structure . Not surprisingly, pharmaceuticals have been appearing in wastewater, surface waters, and in some cases tap water, over the past few years . Standard wastewater treatment facilities are not equipped to completely remove pharmaceuticals , resulting in these compounds being found in effluent. In addition, even higher concentrations of many pharmaceuticals are released during heavy storms in the untreated wastewater overflow, which then directly contaminate the environment . These pharmaceuticals have been found at biologically active concentrations in surface waters around the world . There is also an increasing effort to use reclaimed wastewater in drought-affected areas, such as Southern California . In agriculture/livestock operations, pharmaceuticals are found in manure that is used as fertilizer, effectively compounding the pharmaceutical concentrations . Current research shows these chemicals tend to be both pseudopersistent in soil and detrimental to soil and rhizosphere microbes . Our recent studies of the effects of pharmaceuticals on aquatic insects show that, at concentrations found in reclaimed water, these CECs can alter development of the mosquito Culex quinquefasciatus, its susceptibility to a common larvicide, and its larval microbial communities . Watts et al. showed 17α- ethinylestradiol, a common birth control agent, and Bisphenol-A, a common plasticizer, can cause deformities in the midge Chironomus riparius. However, because larval forms of aquatic insects develop directly in the contaminated water, their constant exposure is likely greater than most terrestrial insects. Interestingly, many CECs, which were not designed specifically to impact microbes, have been shown to affect microbial communities. For example, caffeine, a common mental stimulant, can alter biofilm respiration, and diphenhydramine, an antihistamine,hydroponic dutch buckets has been shown to modify the microbial community of lake biofilms . Due to such unexpected effects, accurately predicting the consequences of specific CECs, even in model insects, is not yet possible.

This problem is exacerbated by a lack of information regarding effects of pharmaceuticals and other CECs on the microbial communities of any terrestrial insects. Arthropods, such as insects and crustaceans, rely on hormones to grow, develop, mate, and produce pigmentation . However, many pharmaceuticals, especially mammalian sexhormones, are structurally similar to chemicals that these organisms rely on for growth and development. These pharmaceuticals then bind to receptors and either over express or suppress their counterparts’ natural function. This has been seen in birds, reptiles, and arthropods where endocrine disruption occurs, primary and secondary sexual characteristics are modified, and courtship behaviors are changed . Although most arthropod hormones do not closely match those of mammals, their molting hormone is very similar in structure to the mammalian female sex hormone 17β-estradiol. In crustaceans, mammalian hormones have been known to cause both increased molting events and inhibition of chitobiase, the enzyme responsible for digestion of the cuticle during insect molting . In insects, 17α-ethinylestradiol, a common synthetic birth control hormone, has been shown to alter molting and lead to deformities of C. riparius . In addition to these effects, pharmaceuticals have been shown to have delayed cross-generational effects . The cabbage looper is a well-studied polyphagous insect native to North America and is found throughout much of the world . T. ni are yellow-green to green in color and can complete their life cycle in as little as 21 d depending on temperature . This species is a pest on many agricultural crops including crucifers and a variety of other vegetables in both field and greenhouse settings .Currently, there is little to no information regarding pharmaceutical effects at the concentrations found in reclaimed water on the growth or microbial community composition of any terrestrial herbivore. Many herbivores can be exposed to these contaminants after the CECs enter surface waters, soil, and plants from wastewater reuse and unintended discharge. To investigate the function of the gut microbes in insects, several studies have used antibiotics applied at high doses . There is also no information regarding effects of CECs when translocated through plants to terrestrial insects.

To test the hypothesis that common pharmaceuticals affect mortality, development, and microbial communities of T. ni, we conducted a series of bio-assays in artificial diet and on a key host plant utilizing surface water concentrations of common important pharmaceuticals. We used a culture-independent approach by performing a 16S rRNA gene survey on both diet and whole-body insects. Any effects would have potentially important implications from agricultural perspectives. Also, as there is currently no information on effects of CECs on terrestrial insects acquired through a plant matrix, our findings would have possible interest for integrated pest management research.In our study, CECs at concentrations found in reclaimed wastewater were shown to increase mortality of T. ni, especially on artificial diets contaminated with antibiotics, hormones, and a mixture of the chemicals. The mortality effect was also evident when T. ni were reared on plants grown in antibiotic-containing hydroponic growth media. Because plants grown in the hydroponic system contained quantifiable levels of ciprofloxacin in the leaf tissue , and the antibiotic treatments significantly changed the microbial community of the insect , we think this is possibly a cause of the mortality but we cannot exclude direct effects of the CECs on the insects or indirect effects through the plants. Ciprofloxacin is a quinolone topoisomerase IV and DNA gyrase inhibitor that acts by stabilizing the DNA-topoisomerase IV and DNA-girase so that it is no longer reversible . This blocks DNA replication and eventually causes cell death of bacteria. However, unlike bacteria, when higher-level organisms evolved, the A and B subunits of the topoisomerases fused, creating homodimers that cannot be targets of ciprofloxacin , and thus damage to the ribosomes of insects is not a possible mechanism of toxicity. Interestingly, we did not see the increased time to adulthood in T. ni reared on plants compared with those reared on contaminated artificial diet. We postulate the discrepancy is possibly due to a number of factors such as dilution of CECs, as they were acquired from the water by the plants or there was bio-degradation of the chemicals occurring in the plant or by photodegradation. However, recent studies have shown pharmaceutical concentrations in surface waters, which appear to remain constant over the course of several years . More studies would be needed to determine how CECs at concentrations found in reclaimed water for agriculture would interact with current IPM strategies , and how soil matrices would affect the chemical acquisition and translocation by plants. Many insects rely on microbial communities and endosymbionts to grow and develop; however, it has been shown that Lepidoptera species do not have a vertically transmitted microbial community .

In addition, because the effects of microbial communities on T. ni survival and development have not been documented, we present these data only to show that microbial communities change when exposed to CECs, and not as a proven factor influencing survival. We found significant shifts in the microbial community in the various life stages examined within the control treatments notably from third instar to subsequent life stages. A similar result has been reported for mosquitoes and other insects . However, there is one family, Lactobacillaceae, which appears in all treatments and life stages in high proportions, except for adults. They are fairly common in insects and can be responsible for at least 70% of the bacterial community . Lactobacillaceae is responsible for ∼42% of the bacteria in all life stages, followed by Pseudomonadaceae, Alcaligenaceae, and Enterobacteriaceae. Lactobacillaceae have been shown to act as beneficial bacteria in Drosophila ; however, its function in T. ni is still unknown. Alcaligenaceae has been shown to be present in other moths ,bato bucket but Lepidopterans are not thought to have a functional microbiome . There are clear patterns regarding the changes in microbial community proportionality according to the heat map . In controls, third-instar microbial communities are relatively evenly spaced by family. The microbial community becomes predominately Lactobacillaceae for sixth instars and pupae. Once the insects reach the adult stage, their most predominant family is Pseudomonadaceae. This pattern holds in the acetaminophenand caffeine treatment groups as well. Interestingly, the other treatment groups do not share this pattern. For antibiotic- and hormone-treated T. ni, Lactobacillaceae is the predominant microbial family in the immature stages, but at the adult stage microbial community reverts to predominantly Pseudomonadaceae. We suspect that this is because, once the larvae undergo metamorphosis and shed their gut contents in preparation for pupation, they are no longer exposed to the pressures exerted by the CECs on the microbial community. Fig. 3 provides a visual indication of the changes in the bacterial communities over time. The increase in β diversity after eclosion could be due to the larvae no longer being exposed to CECs or diet-borne bacteria after being moved to sterile containers. Also, when bacteria are lost as larvae digest their gut contents during pupation, the microbial β diversity could change. Interestingly, the hormone-treated T. ni follow a similar pattern to those exposed to antibiotics, but their ellipses are always much smaller, suggesting the entire insect population is showing a uniform response within their microbial communities. However, in the mixture-treated insects, larvae displayed a greater average diversity in their microbial community structure than either pupae or adults. This finding has not been shown in any single category of treatment, and we suspect the microbes exposed to mixtures could be experiencing potential interactive effects among chemicals . Such interactions should be the focus of future studies along with investigations of plant rhizosphere bacteria, particularly since we found a difference in the Bradyrhizobiaceae family for all treatments. These results show that a terrestrial insect pest of commercial crops can be affected by CECs found in reclaimed wastewater for agricultural use. Our results suggest that CECs found in wastewater can impact T. ni growth and development, survivorship, and alter their microbial communities. Because T. ni is a common agricultural pest found around the world, feeds on a wide variety of plants, and has a history of developing pesticide resistance, its ability to deal with toxins is likely higher than many other insects. In addition, the responses we observed to CECs could have interesting implications for IPM practices on plants such as lowering the amount of pesticides needed or increasing susceptibility to insect pathogens, as has been shown in mosquitoes . These potential effects may be understated because some insects cannot detect the presence of the pharmaceuticals . However, we do not recommend purposefully exposing crops to CECs specifically for the control of insects because our study documented that these pharmaceuticals are translocated into crops and we do not yet know their possible effects on humans if consumed . We specifically want to note that ingestion of these compounds through uptake and translocation by a plant is not the only way T. ni or any other insect would be exposed to these compounds. Overhead sprinkler irrigation could cause contact absorption by the plants or insects, and simply drinking water on leaves at contaminated sites could expose insects to higher concentrations than were found in plant tissues. In fact, the ciprofloxacin concentration used was less than one-third of the highest rate . We urge caution in extrapolating to plants growing in soil, because variation in soil type and potential soil bacterial degradation could affect persistence [although soil bacteria are often negatively impacted by CECs ]. However, CEC exposures are considered pseudopersistent because they are reapplied with each irrigation. Thus, the effects reported here are likely to be conservative. Additional studies with other insects, particularly those with other feeding strategies, will be necessary before any patterns can be discerned.The growth of the human population places an ever-increasing demand on freshwater resources and food supply. The nexus of water and food is now well recognized. One promising strategy to sustain food production in the face of competing water demands is to increase the reuse of treated human wastewater. Municipal wastewater reuse for food production has been successfully adopted in some regions of the world. For example, Israel uses ~84% treated wastewater in agriculture production .

Each offset credit is equal to one metric ton of carbon dioxide equivalent

Offset programs make cap and trade programs “more attractive and palatable” to covered entities, as offset programs provide more flexibility to determine the lowest-cost method of reducing greenhouse gas emissions.As long as an offset project will be cheaper than internally reducing emissions, capped entities will likely seek out credit for emission reductions through offset programs. Offset programs are beneficial for governments implementing cap and trade programs because it shows that they are trying to work with industry to find lower cost means of reducing emissions to the mandated level. Additionally, now that offset programs are widely implemented, it would likely be more difficult to gain support for a cap and trade program that did not include offset programs. Offset programs are also beneficial for sectors that are target hosts for offset projects because offset projects are a source of improvements and an income opportunity for the host.Typically, the project host receives financial incentives or some sort of technology, facilities, or practice upgrade that they could not afford or would not have undertaken otherwise. Thus, the benefits of offset programs not only affect the capped entities, but also sectors that are otherwise unaffected by the cap and trade program.In the United States, the U.S. Environmental Protection Agency estimates that agriculture accounts for 8% of the country’s greenhouse gas emissions.The EPA estimates that half of these agricultural emissions come from management practices of agricultural soils, including fertilizer application, irrigation, and tillage methods,hydroponic nft and that livestock manure management accounts for 15% of the agricultural emissions.The livestock digestion process accounts for about one third of the agricultural emissions and the remainder comes from smaller sources, such as rice cultivation and burning crop residues.

These estimates do not include carbon dioxide emissions from on-farm energy use.CARB’s Scoping Plan estimates that the agricultural sector contributes to about 6% of the total greenhouse gas emissions in California.CARB also includes estimates of emissions based on the end use rather than the actual source of emissions.When calculated in this manner, 9% of California’s greenhouse gas emissions can be attributed to agriculture and food processing industries.In general, the agricultural sector provides at least two strong avenues for reducing greenhouse gases through offset programs: decreasing emissions from raising livestock and sequestering carbon in agricultural soil.California has already incorporated an offset program that takes advantage of the opportunity to decrease livestock emissions by installing biogas control systems , which capture and destroy methane, on dairies and swine farms.An offset program that takes advantage of the second opportunity to decrease carbon concentrations in the agricultural sector by sequestering carbon in agricultural soil has been used in conjunction with other cap and trade programs and may provide an opportunity for an expansion of California’s offset programs in the future.Many livestock operations manage livestock waste by using anaerobic liquid-based systems in lagoons, ponds, tanks, or pits.61 Manure that is stored in this fashion emits methane,a powerful greenhouse gas that is estimated to have a radiative forcing power, or global warming potential, twenty-five times that of carbon dioxide.Manure management accounts for 15% of the agricultural greenhouse gas emissions in the United States, and CARB’s most recent estimates indicate that manure management accounts for 1% of California’s total greenhouse gas emissions.Even though manure management is not the largest source of agricultural emissions, California’s cap and trade program includes the Livestock Projects Compliance Offset Protocol , an offset program that issues offset credits in exchange for installing biogas control systems , a type of manure digester, on dairies and swine farms.BCSs capture methane from the livestock operation’s manure storage facility before it is released into the atmosphere.The Livestock Protocol permits the captured methane to be destroyed on-site, transferred offsite, or used to power vehicles, but “the ultimate fate of the methane must be destruction.”

The dairies and swine farms may then sell the offset credits that they produce through this offset program on AB 32’s carbon market.The Livestock Protocol is considered a standards-based offset protocol, as it “creates additionality thresholds for particular categories of projects instead of determining additionality individually for each project.”CARB’s standards-based approach for its current offset protocols came under attack in Citizens Climate Lobby et al. v. California Air Resources Board.70 Citizens Climate Lobby argued that CARB’s standards based approach results in non-additionality by issuing offset credits for greenhouse gas reducing projects that would have been completed anyway, which impermissibly enlarges the scope of AB 32, and that CARB should have adopted a project-byproject approach instead in order to perfectly determine whether each offset project is indeed additional to business-as-usual.In addition to determining that using a standards-based approach for offset protocols was within CARB’s authority, the court in Citizens Climate Lobby determined “that the Livestock Protocol is reasonably necessary to effectuate the purpose of [AB 32] and [CARB] was neither arbitrary nor capricious in its promulgation.”The court made this determination by reviewing evidence presented by CARB that less than 1% of dairies and swine farms in the United States install BCSs to dispose of manure, installing BCSs is not a standard or common practice, and that the cost of installing a BCS is the primary barrier to installation.Because farms were not installing BCSs despite other favorable conditions for installation, the court stated that it is not arbitrary for CARB to use installation as the standard to determine additionality.Thus, CARB maintains a standards-based approach, rather than a project-by-project approach, for its Livestock Protocol. The court reached a similar decision regarding CARB’s three other offset protocols.Agricultural soil carbon sequestration offset programs function like other offset programs, but the projects can include switching to conservation practices including no till, conservation tillage, planting cover crops, utilizing high-diversity crop rotation, and other agricultural practice changes in order to increase the amount of carbon sequestered in the agricultural soil and reduce the amount of emissions from farm machinery.

All of these practice changes increase carbon sequestration by differing amounts. The U.S. Environmental Protection Agency estimated that conservation tillage can sequester between .6 and 1.1 metric tons of carbon dioxide per acre per year.The U.S. Department of Agriculture estimated that planting cover crops and improving crop rotations and fallowing practices can sequester between .2 and .4 metric tons of carbon dioxide per acre per year.78 One assessment of the effects of conservation practices on cropland in the Missouri River Basin estimated that the studied area sequesters 9.9 million tons of carbon dioxide per year.Estimates of the global potential of soil sequestration vary greatly, but one estimate says .9 petagrams of carbon per year may be sequestered globally, which is enough to offset one-fourth to one-third of the annual global increase in carbon dioxide concentrations.Soil’s sequestration properties occur naturally when organic compounds produced by plants cycle through plants, animals, and microorganisms to create soil organic matter,hydroponic channel which is where carbon is stored in the soil.Carbon is released from the soil into the atmosphere when it is disturbed due to changes in water, air, and temperature conditions.Thus, reducing tillage increases the carbon sequestered in the soil, and the level of sequestration depends on many variables including soil type, weather, precipitation, temperature, and other factors. Aside from decreasing atmospheric carbon levels, the practices that encourage carbon sequestration boast local benefits such as reducing soil erosion and nutrient depletion while increasing water retention rates.A USDA project that ran from 2003-2006 assessed the effects of cropland conservation practices, including tillage management along with a host of other conservation practices that also sequester carbon, in the Missouri River Basin.84 The assessment determined that conservation practices decreased loads delivered from cropland to rivers and streams by 76% for sediment, 54% for nitrogen, and 60% for phosphorous.85 These dramatic reductions cannot all be attributed to changes in tillage management or other carbon sequestering practices, as those were only some of the measures among many diverse strategies for decreasing sediment and nutrient loss from agricultural soil. Additionally, the assessment noted that carbon that is sequestered in agricultural soil “improves the soil’s ability to function with respect to nutrient cycling, improves water holding capacity, and reduces erodibility through enhanced soil aggregate stability.”So, in addition to sequestering carbon, the conservation practices that are typically implemented in agricultural soil carbon sequestration offset projects provide many important benefits incidental to sequestering carbon.The EU ETS, the Kyoto Protocol, and RGGI, some of the most major carbon markets in the world, currently do not recognize offset credits that are generated from soil carbon sequestration projects.This is most likely “because soil carbon is viewed as difficult to measure, verify, and track.”88 However, some smaller markets recognize this opportunity for carbon sequestration and income for farmers, so some offset programs that generate credits for agricultural soil carbon sequestration are already in existence. In 2010, the World Bank implemented the Kenya Agricultural Carbon Project, which encourages “covering crops, crop rotation, compost management, and agro-forestry.”This method of farming generates credits that are sold to the World Bank’s BioCarbon Fund.Additionally, in 2012, the World Bank created a new farming methodology, approved by the Verified Carbon Standard,that encourages less plowing.Before the Chicago Climate Exchange’s closure in 2010, it verified and traded soil carbon offset credits generated by farmers in the United States using no-till practices.

The Oklahoma Carbon Program currently operates a voluntary program that verifies and issues credits for farmers who use conservation tillage.Canada’s guidance for its future offset programs indicates that it would include an agricultural soil carbon sequestration offset program to address the intensity of tillage operations, adopting crop rotations, and increasing cover crops.If the American Clean Energy and Security Act of 2009, better known as the Waxman-Markey bill, had been approved by both houses of the United States Congress, it is likely that agricultural soil carbon sequestration offset programs would have played a role through that legislation’s proposed nationwide cap and trade program.The agricultural industry was concerned that other industries’ products used by agriculture—fertilizer, diesel, electricity, etc.—would increase in price if the proposed legislation passed, in turn affecting the agricultural sector’s expenses.The National Corn Growers Association devised nine principles relating to cap and trade that had to be met before it would support any climate legislation bill.The first principle is that “[t]he agricultural sector must not be subject to an emissions cap.”The second principle asks cap and trade to “fully recognize the wide range of carbon mitigation or sequestration benefits that agriculture can provide.”The fourth principle expects the USDA to create the regulations and oversee an agricultural offset program.The fifth principle provides that “[t]he use of domestic offsets” is not “artificially limited.”This principle is directly at odds with current caps on offset credits that can be used to meet compliance obligations as in RGGI and AB 32’s cap and trade program. Additionally, the Illinois and Iowa Corn Growers Associations owned Novecta, a group that was working on standardizing a program to reward farmers for no-till practices.Agricultural soil carbon sequestration offset programs were also proposed and discussed in relation to the Lieberman-Warner bill, the cap and trade climate change bill that was introduced in the 110th Congress, just before the Waxman-Markey bill was introduced in the 111th Congress.Considering this significant support for agriculture offset programs, it is likely that an agricultural soil carbon sequestration offset program could have been implemented on a nationwide scale if Waxman-Markey Bill had passed. Overall, agricultural soil carbon sequestration offset programs prove to be attractive because the farmers implementing the change are paid to change their practices.This can be a welcome source of income, especially at a time when farmers, especially small-scale and those most affected by droughts and the changing climate, are having a difficult time maintaining productivity and income.Considering all the factors discussed above, it seems that a future natural step may be to adopt an agricultural soil carbon sequestration program to link to AB 32’s cap and trade program. The possibility has already been recognized in a bill that was proposed to the California State Assembly. The proposed bill stated that new offset programs will be needed in order to supply the highest number of useable offset credits allowed under AB 32.An early version of the proposed bill listed possible offset programs, including offset programs that maintain agricultural productivity while emitting less greenhouse gases—the idea behind soil carbon sequestration offset programs.

Pests and diseases are another uncertainty for which little published literature exists

Of the five initial variables, the fraction of land in cropland, the soil Storie index, and the land fraction converted to urban had high positive loading values on PC1. The close relationship between these variables is consistent with other studies that show high rates of urbanization on some of the highest quality cropland in the state . Soil salinity and the fraction of land in the 100‐yr floodplain had high positive loadings on PC2. Figure 2.4 shows the spatial distribution of land use vulnerability throughout California as measured by the sub‐ index. While relatively high land use vulnerability occurs throughout the Central Valley, areas of particular concern are the Sacramento‐San Joaquin Delta, and the corridor between the Sacramento and Fresno. In these areas of rapid change from agricultural to urban land uses, sub‐index values were frequently > 2.5 standard deviations above the mean. In the Delta region, the high vulnerability was largely due to the risks posed by both urbanization and flooding on highly productive agricultural soils. In contrast, a combination of increasing urbanization and high soil salinity were the important drivers of vulnerability further south in the San Joaquin Valley. Conversion of prime farmland to urban uses is essentially a permanent loss of agricultural potential, with many consequences for agricultural livelihoods and society at large. When urban development fragments agricultural land, farmers often lose the benefits associated with being part of an integrated farming economy; for example, sources for inputs, information sources, and processing facilities . Farming activities occurring along the urban edge can raise concerns about noise, odor, dust, and spray drift among new suburban residents, while vandalism of farm fields can cause problems for farmers . Regional and local strategies to preserve farmland and manage urban growth include strengthening agricultural zoning policies,hydroponic gutter acquisition of conservation easements on farmland, establishment of urban growth boundaries, and prioritizing infill development .

Given that greenhouse gas emissions from urban land can be more than 70 times greater per unit area than cropland , policies that preserve agricultural land will also help achieve the mitigation targets set by California’s recent suite of climate policies, namely AB 322 and SB 375.3 While the risks of flooding and soil salinization are not new to California farmers, they are likely to be exacerbated by climate change. Declining snow water storage in the Sierra Nevada is expected to increase the frequency and severity of flooding in the Central Valley . As such, efforts to help regional and district water resource managers develop accurate flood forecasts and flexible reservoir operations will further improve adaptive capacity .More than 3 million acres of irrigated farmland in California have soils with an electrical conductivity above 4 dS m‐1, a standard threshold for the occurrence of agricultural impacts . Of the acreage affected, more than two‐thirds is located in the San Joaquin Valley. In these areas, various irrigation methods can be used to leach salts out of the crop’s rooting zone . But since salts can still accumulate along the margins of the wetted area, growers must often apply water in excess of crop needs to ensure that salts are sufficiently leached . The installation of systems to drain, reuse, and dispose of saline effluent are also options, though high costs and a lack of suitable disposal sites remain important barriers .Results of the PCA for the socioeconomic vulnerability sub‐index indicate that 70.3 percent of the cumulative variance among grid cells is accounted for by retaining three principal components . Seasonal and migrant farm workers, loss of farms, and farm disaster payments all had high positive loadings on PC1, while loss of farm jobs and the social vulnerability index loaded highly on PC2. The commodity concentration was largely independent of these other factors, as indicated by its high positive loading on PC3. Three counties along California’s Central Coast all had socioeconomic sub‐index values greater than 1.5 standard deviations above the mean . The high vulnerability of these counties was due to two main factors: the high rate of disaster payments per unit of cropland; and the large number of seasonal and migrant farm workers per unit of cropland. A closer look at the agriculture in these counties reveals that while each have only a small amount of cropland, the mild coastal climate allows them to devote a large fraction to vegetable and berry crops. Since these tend to be high‐value crops that require more labor, it follows that disaster payments and the number farm workers per unit of cropland area are also higher.

Larger counties such as Monterey, San Joaquin, Imperial, and San Bernardino had moderately high socioeconomic vulnerability due to some of the same factors. In Yuba, Sutter, and Madera counties vulnerability was driven by a combination of high disaster payments and a loss of farm jobs. The main factor influencing the high vulnerability in Mendocino County and the moderately high vulnerability in Napa and Sonoma counties was their high Herfindahl index values, which captured the heavy concentration of wine grape production in this region. While disaster payments are used here as an indicator of vulnerability, the federal programs that provide these payments are generally seen as a way to help farmers cope with risk and strengthen their adaptive capacity. Since many fruit and vegetable crops receive no federal subsidies, disaster payments and crop insurance are among the few remaining options for specialty crop producers . However, as agricultural support programs receive greater scrutiny under tightening state and federal budgets, studies that examine the impact of potential reforms and their effects on vulnerability are needed. In contrast to government programs, the advantage of diversification to new crops, products, markets, or income sources is that farmers have more control over the outcome. But while diversification can help spread risk and facilitate a shift toward new crops should the need arise, concerted efforts to improve knowledge‐sharing among stakeholders will be needed to overcome the risks and trade offs associated with unfamiliar cropping systems and market opportunities .Figure 2.6 provides an illustration of total agricultural vulnerability statewide by integrating the four sub‐indices into one total AVI index. Based on this analysis, moderate vulnerability exists in most of California’s agricultural lands, which suggests that there is a need for all agricultural communities to begin to develop adaptation plans that address the potential impact of changing climate, land use and economic factors. Many local and regional governments are now developing climate action plans that accompany updates to their general plans . To date, these climate action plans have mostly focused on greenhouse gas mitigation, but the results presented here suggest that adaptation should hold an equally important place in local planning activities. The total AVI also suggests that there are several regions of concern that merit careful consideration.

These include the Sacramento‐San Joaquin Delta, the Salinas Valley, the corridor between Merced and Fresno, and the Imperial Valley, which all had a mix of high and very vulnerability. While the sub‐indices discussed above help to highlight the location‐specific factors contributing to these regions’ overall vulnerability, the indexing method used in this study is inherently coarse. Given this limitation, future studies that follow a “place‐based” approach will be needed in order to understand the unique local characteristics, both biophysical and socioeconomic, that may contribute to improved resilience within agricultural communities. The recently completed case study of agricultural adaptation to climate change in Yolo County, summarized in Section 3 below, is an early example of how to integrate these elements .While the AVI presented above represents an early a proof of concept, significant gaps remain in the set of potential variables that could be included in the index. In particular,u planting gutter future iterations of the AVI will need to consider additional variables that more fully assess the vulnerabilities to California’s water resources and livestock systems in a spatially explicit manner. For livestock, studies that evaluate statewide spatial variation in the season length of adequate forage and its links with winter precipitation may be a useful addition . These are but a few of the many types of spatial datasets that might be integrated in to the California AVI. In its current form, the AVI is designed to assess “present” agricultural vulnerability. However, going forward there is potential to modify the AVI so that it can accommodate future projections of climate, land use, and socioeconomic variables. For example, integrating down scaled climate projections into the climate vulnerability sub‐index, or integrating statewide UPlan runs into the land use vulnerability sub‐index, are very feasible next steps . Yet, since many of the biophysical and socioeconomic factors included in the sub‐indices can vary unpredictably over time, and in some cases have not been accurately modeled into future, use of the AVI to examine future scenarios may have inherent limitations. To overcome the potential limits, contributions of expertise and data from a broad range of stakeholders, government agencies, and academic disciplines will no doubt be required.Preservation of agricultural land is a priority in Yolo County, and planning is focused on regional land use guidelines that maintain land in agricultural production and concentrate new development into urban areas. Regions within Yolo County are distinguished by their land forms , proximity to the Sacramento River and Delta , water availability , and the influence of small towns and cities . There is greater prevalence of wine grapes along the river, processing tomatoes in the alluvial plains, and organic fruits and vegetables in an isolated, narrow valley to the north. Flooding along the Sacramento River poses the most significant regional hazard from climate change; water flows will increase by at least 25 percent by 2050 due to a decrease in snow pack in the Sierra Nevada . As for most of California during the past few decades, there has been a trajectory toward less crop diversity, larger farm sizes, but fairly stable markets for commodities . Most commodities are managed with high intensification of agricultural inputs . The number of organic farms, however, is growing. A recent survey showed that many riparian corridors have low scores for soil quality and riparian health , and there is concern about transport of pesticides to the San Francisco Bay delta .

Environmental quality is now receiving more attention with active grower participation in programs from several agencies.Phase I of this case study examined possible effects of increased temperature and decreased precipitation on Yolo County crops . The horticultural “warm‐season” crops in the county will experience more stress than field crops, due to greater environmental sensitivity of their reproductive biology, water content, visual appearance, and flavor quality. New horticultural crops may include “hot‐season’ crops in summer, and “cool‐ season” crops that prefer warmer winters. Expansion of citrus and of heat and drought‐tolerant trees are likely partly because fewer winter chill hours will be difficult for some stone fruits and nuts . Forage production for livestock in upland grasslands may increase with warmer temperatures during the winter rainy season, but field experiments with elevated carbon dioxide do not corroborate this expectation . More nitrogen limitation will likely occur under eCO2 , unless N‐fixing legumes become more abundant. During the past 25 years, crop diversity has decreased across Yolo County , but resilience to extreme events, such as heat waves, may be enhanced in the future by a more diverse crop mix that varies in stress tolerance. Water supply has been considered the most uncertain aspect of climate change for farmers in Yolo County, who rely on groundwater for about 30 to 40 percent of their supply in a normal water year .Discussions with the Yolo County UC Cooperative Extension farm advisors indicate special concern for stripe rust on wheat , insectpests on nuts, medfly, corn earworm on tomato, tomato spotted wilt virus, stem nematode on alfalfa, and earlier activity of perennial weeds such as bindweed . Crop management is subject to change to improve production and environmental quality. Phase I evaluated a set of practices and found that most practices either benefitted GHG mitigation or benefitted adaptation to a changing climate. More comprehensive analysis of these complex relationships is needed.

Farmers can respond by shifting their production into less labor-intensive crops

However, domestic food demands continue to increase and diversify, creating important employment opportunities in the off-farm AFS. These changes mean that both traditional and new digital technologies can be leveraged to induce a productive exit out of agriculture in Sub-Saharan Africa while maintaining a competitive agricultural workforce on and off the farm in the chains elsewhere. Three key policy implications emerge. First, productivity-enhancing investment in agriculture must accelerate in the lower-income countries and proceed at least in tandem with the movement of workers off the farm elsewhere. Populations will continue to grow despite slowing birthrates, and food production will have to expand to keep pace. The movement of workers off the farm to meet the demand for other goods requires producing more food with fewer workers, once underemployed labor has been activated. Historically in today’s high-income countries, agricultural extension and public investments in infrastructure, from irrigation to information, marketing institutions, and roads, played a critical supporting role in facilitating the labor exit out of agriculture. They enabled the remaining farmers to earn a living commensurate with non-farm sectors, as competition for workers with the non-farm sectors and downstream food processors intensified. This agenda holds as much today as then. In Sub-Saharan Africa, the agricultural share of public spending continues to be well below that in East Asia . Myriad input, factor, and output market constraints hold agricultural labor productivity back, and integrated solutions that simultaneously overcome a number of these constraints are needed. Inclusive value chain development ,dutch bucket hydroponic which links farmers with buyers in contracting arrangements, offering knowledge, access to credit and inputs , and higher prices in exchange for a consistent volume of high-quality products , provides a market-based solution to do so, though smallholders’ lack of legal protections can be an obstacle .

Given the challenge to develop self-enforcing incentive compliant contracts, iVCD typically does not work well for raising staple crop productivity. Yet, in low income countries, this is where the need and scope for raising labor productivity and poverty reduction is highest. For raising labor productivity in staple crops, more and better public investment in public goods is needed . Second, the scope for iVCD to raise smallholder incomes and benefit the poor and women is greater for non-staples. iVCD also creates jobs off the farm, in the chains and beyond . Success factors of iVCD include careful diagnosis of the competitiveness and sustainability of the product value chain chosen, starting small, involving financial institutions, monitoring producer-buyer relationships, and sustaining capacity building. This is in addition to creating an economic environment that is conducive to investment generally. Developing systems to monitor and enforce food quality standards in the AFS is equally critical. There is clearly a role for agricultural ministries, as well as for the private sector, to ensure that the development and use of labor-saving technologies keeps pace with the movement of workers off-farm. Many questions remain, however, especially on the best entry points for support: through farmer organizations/cooperatives, large anchor firms and/or SMEs, or externally initiated stakeholder platforms. More experiments are needed. In the meantime, appropriate measures will be needed to help SMEs in the transformative food chains see through the decline in liquidity caused by COVID-19 and avoid undue concentration of activity in the long run. Labor-market regulations and other social protections can also be useful in protecting vulnerable populations from exploitation as they transition into non-farm work . Third, investment in people is critical to raise agricultural labor productivity and to make sure that those leaving can access the new jobs in the AFS, as well as other non-farm sectors, and meet the rising economic aspirations of rural youth.

Continued investment in quality rural education, which continues to largely underperform in developing countries, is needed . Increasing educational attainment in rural areas facilitates technology adoption, as well as occupational mobility, and reduces income inequality. This is also important for young women facing social norms that make it difficult to escape from traditional gender roles. Nontraditional skill-building programs and effective agricultural extension systems will be equally needed to build up human capital in regions where traditional education has proven ineffective. The extension system is particularly weak in Sub-Saharan Africa and has been largely neglected for the past couple of decades by governments and donors alike. The 2010s have witnessed a surge in studies on social network or farmer-to-farmer technology extension, which proves more promising especially in combination with public extension than traditional public-sector extension approaches. But several issues remain such as the choice and compensation of appropriate lead farmers . Policy implications are different, but just as immediate, in high income countries. Rich-country farmers will be required to produce more and higher-quality fresh and processed foods for a growing, and increasingly affluent, domestic and global population, and they will be required to do so under increasingly stringent environmental and animal welfare standards. However, they will have to do this with fewer workers. The transition of domestic workers out of farm work largely has run its course in rich countries. The option of importing foreign workers is gradually closing, due to a declining farm labor supply in farm labor exporting countries and a less supportive political environment for immigration, particularly of low-skilled workers, in high-income countries. Three key policy implications emerge for high-income countries in this era of growing farm labor scarcity: First, farmers in high income countries will increasingly need to look beyond immigration policy as an answer to farm labor scarcity —especially in the medium and long run.

Guest worker programs can expand as a short-run response to farm labor scarcity. However, as the structural transformation progresses in farm labor-exporting countries and political resistance to importing low-skilled farm workers intensifies, the immigration solution to the farm labor problem becomes less of an option. This does not mean that immigration will not continue to play a central role in farm labor markets throughout the developed world for some time. But farmers will need to take steps to retain an aging, mostly immigrant, workforce while pursuing available options to contract new workers from abroad. International farm labor migration could continue to be a much-needed channel for sharing prosperity across nations and reducing poverty in the world’s poorest countries. For this, however, a counternarrative needs to take hold rapidly. If not, its days may be numbered prematurely, especially now that the COVID-19 pandemic so clearly exposed the agri-food sector’s dependence on immigrant labor and the logistical challenges this may entail, eroding support for reliance on immigrant agricultural labor even further. Second, increasingly sophisticated technological change is going to be a fundamental feature of the food supply chain,dutch buckets system from farming to food processing. Productivity-enhancing investments likely will include the use of highly-advanced robotic systems that will dramatically reduce the need for workers . Scouring the landscape in today’s high-income countries, we find automation success stories like the ones described earlier in this paper, as well as major challenges. There is a danger that automation will not happen quickly enough to enable farmers to maintain their competitiveness in a high wage, labor-scarce, world.However, more affluent consumers will demand fresh, locally-grown fruits and vegetables, as well as specific qualities like organics, environmentally friendly production practices, fair trade, and possibly better labor practices, all of which tend to increase labor demands compared to field crops where automation is more advanced. Prices of these fresh fruits and vegetables will rise, causing farmers to think twice about abandoning production as wages rise while intensifying pressure on public and private researchers and policy makers to accelerate the development of labor-saving technologies and deploy the necessary digital infrastructure to run it, including in remote rural areas.

Policymakers will need to keep an eye out for undue concentration of power in the supply of these new technologies and devise adequate policies to ensure competition . Third, a technologically advanced AFS requires a technology-savvy workforce, with more engineers and people capable of working with increasingly complex technologies. As agricultural and food processing technologies become more IT intensive, so do human capital demands all along the AFS. To some extent, developments in IT can help respond to human capital shortages; viz. bar codes in supermarkets and hamburger buttons at fast-food restaurants. Nevertheless, the numbers of workers with little education who pick themselves a living wage will diminish. As new technologies become available for relatively easy-to mechanize crops and routine tasks, the farm workforce will move out of those crops and tasks into ones that have not yet been mechanized and are non-routine . A major policy challenge is to prepare the future farm workforce for technological change while also ensuring that employment opportunities expand as new technologies release workers from crop production. There is no magic bullet to guarantee that automation, human capital formation, and new job creation move apace. It is undeniable that the future holds far-reaching changes in mechanization and automation in developing and developed countries alike. Without it, agriculture and the AFS generally will not be able to keep up with rising food demands and a declining farm labor supply. Inevitably, many farms and farm workers will have difficulty adjusting. Some farms and farmers, particularly larger, wealthier and better educated ones, are in a far better position to experiment with and adopt new labor-saving technologies, including advanced robotics. And some farmers and farm workers, particularly older ones, will have a difficult time shifting to new commodities and tasks; the more technology-savvy farm workforce of the future is likely to be younger and better educated than current workers. Decoupling social insurance from employment, as proposed in Packard et al. , could be a worthwhile social insurance model to mitigate adverse consequences of this transition and avoid the introduction of ineffective agricultural and food policies. The need for greater food system resilience, highlighted by the COVID-19 experience, would also be better served by food trade diversification instead of a reversal to protectionism and food self-sufficiency. Yet, without successful social insurance schemes to help mitigate the adjustment costs and rapid ramp up in agricultural education and extension, the ongoing evolution in the agricultural labor force is bound to raise inequality as well as anti-trade sentiment, including in agri-food. Agricultural production has grown to meet the demands of an increasingly large and wealthy human population. The development of high-yield crop varieties combined with the widespread use of irrigation, synthetic fertilizers, pesticides, and land use changes that marked the “Green Revolution” have enabled an enormous increase in crop production per area . As a result of these technologies, cereal production has doubled . This increased production is credited with reducing poverty and improving nutrition intake for millions of people worldwide . However, this increase in production also has costs. There are concerns that the loss of natural enemies and biodiversity caused by the increased size and connectivity of agricultural land, the trend toward monocultures, and the conversion of natural habitat—termed “landscape simplification”—makes farms more susceptible to pest outbreaks . With increased risk of pest outbreaks comes enhanced pesticide use. Although other aspects of intensive farming also have negative externalities, such as synthetic fertilizers and eutrophication, pesticides have received some of the most widespread scrutiny and their reduction has become a priority for policy makers, as evidenced by integrated pest management . The emphasis on pesticide use stems from serious human health concerns related to pesticide exposure in farm workers , pesticide residues in food and water sources , and bio-accumulation of pesticides in higher trophic levels . Despite popular ecological thinking that the connection between landscape simplification and pesticide use is clear, both theoretical and empirical studies have found ambiguous results. Agroecological theory holds that landscapes composed of a high proportion of cropland are more susceptible to pest outbreaks because of their habitat homogeneity and reduced natural enemy populations. Therefore, more simplified landscapes would experience more pest problems and consequently use more pesticides. Conversely, economic theory of pesticide use suggests that the application of pesticides by a neighboring farm may have positive externalities for surrounding farms as a result of pesticide drift or pest suppression .

Increased reliance on groundwater resources makes evaluation of new potential sources a priority

While this choice can be painted as paying the class in question not to work and thus may face push back from the public or even within government, it avoids the myriad complications that stem from subsidizing production, including surplus crises, fights over price adjustments, and conflicts with international trade agreements. In short, leaving production to the most efficient, while supporting the less competitive via income payments allows policymakers to open up more market share for the most efficient while protecting the uncompetitive from impoverishment. A second critical juncture concerns rules structuring benefits, specifically the imposition of benefit limits. A decision to forego benefit limits is arguably more inclusive as it does not discriminate based on some measure of success or size of operation but is also more expensive. Alternatively, the decision to restrict benefits can be seen as discriminatory, splintering the target group into the privileged and unprivileged while saving costs in the long run. This decision has important consequences for the scope and type of future reform. Despite repeated attempts, a reform that the CAP has never been able to impose due to ardent opposition from a few member states is a benefit limit. As a result, some farmers receive hundreds of thousands of Euros in CAP income payments every year. Policymakers wishing to minimize the costs of support to extent possible will want to impose benefit limits, whether they be yearly or lifetime, at the time the policy is created. Benefit levels can always be extended and expanded, but it is much harder to retrench these policies. By imposing limits early, policymakers can better contain overall costs. In addition, these choices position politicians to make popular reforms rather than unpopular,nft hydroponic and likely unsuccessful reforms . However, it may be quite difficult to get the target’s representative groups and unions to agree to a policy that essentially imposes a two-tier system.

The third critical decision concerns qualification and/or behavior standards and requirements. On the one hand, avoiding or limiting standards and rules increases the odds of compliance and support from the target population. On the other hand, imposing standards and rules early controls costs and makes future reform easier. The CAP has struggled to evolve over time, largely failing to impose even the most basic of standards on payment recipients. Policymakers who decide to manage class decline through a CAP-style income support system must think carefully about what types of standards and requirements make sense and impose them early. For example, two reforms CAP officials have struggled to impose are environmental standards and eligibility rules . Neither of these ideas, that farmers should have to meet basic environmental standards or that one’s primary profession must be agricultural in order to receive aid for those employed in agriculture, is particularly radical, but since no real rules or standards for good environmental practices or eligibility existed when the CAP was created, it has been nearly impossible to impose them in a meaningful way in subsequent reforms. Taken together, these three junctures identify moments when policymakers must think carefully about their decisions for how to manage a declining class because these decisions are sticky, proving hard to reverse, and carry important long-term implications. My dissertation and its core findings speak to a broader question of what happens to declining social classes. By investigating the current status of farmer power, I cast light on how and when the political power of a social class is affected by a decline in numbers. Farmers are not the only class to have experienced a dramatic reduction in its share of the population; the blue-collar industrial working class has shrunk dramatically with the shift to a service sector oriented economy. European governments have buffered workers against the effects of deindustrialization with generous disability and early retirement benefits. In addition, in many countries, unemployment programs for workers were structured to ensure that benefits would not run out. As this brief example illustrates, the framework I have used to examine the decline of Europe’s farmers can also be deployed to examine and explain the decline of other social classes. For both blue-collar workers and farmers, policy took the same path. It started with an effort to preserve the class by subsidizing employment and ultimately ended up with a policy that paid individuals not to work.

The path to this final policy outcome in both cases was long and expensive. The lesson, then, for reformers is move to an income support policy as quickly as possible. While these policies are expensive and often unpopular, costs can be contained and crises avoided if these types of programs are adopted first, and reasonable benefit restrictions are imposed early. In sum, my project is not only about the intricacies of CAP reform, but also about the conditions that permit or forestall EU and welfare state policy reform, the techniques for overcoming resistance to policy change, and ultimately the politics of and strategies for managing social class decline. This body of research beginning with my study of farmers and expanding to other threatened social classes will further clarify the important puzzle of why some groups are able, against all odds, to exercise strength without numbers.The rising prevalence of drought conditions in California and elsewhere has dramatically increased demands on groundwater for irrigation and human consumption.Organic and inorganic contaminants in the water supply are prevalent in many human impacted sites such as agricultural, industrial, and municipal. However, simply measuring known sources of contamination has the potential to miss the complex effects of microbial communities in the soil and groundwater. Diverse microbial communities in subsurface environments including groundwater systems exhibit extraordinary phylogenetic diversity and metabolic complexity that has only recently become apparent using culture-independent sequencing-based analytics. The impact of changes in water chemistry on these aquifer microbial communities, and ultimately on groundwater quality, is unknown. Nitrogen as ammonia and nitrate are among the most ubiquitous groundwater contaminants due to widespread use in agriculture as fertilizers, as unintentional discharge in septage and effluent. While crops absorb much of the applied fertilizers, significant amounts leach to groundwater. In certain regions of California’s Central Valley, over 40% of drinking water aquifers have elevated levels of nitrates. The impact of these nitrogen compounds on environmental and groundwater microbial communities is not well understood, including the secondary effects on human, livestock, and wildlife health, and the potential for naturally occurring microbial populations to mineralize ammonia and nitrate to non-toxic forms. There thus exists an urgent need to understand these processes and how they may interact with remediation strategies to protect the quality of groundwater supplies.

To explore these important issues, we sampled groundwater from three adjacent wells completed at different depths that are part of a long term study on agricultural groundwater. The wells are affected to different degrees by manure, a common source of aqueous agricultural contamination. We subjected these samples to chemical analytics as well as next-generation sequencing, assembly,nft system and genomic analysis. Our genomic analysis revealed a highly diverse microbial community dominated by many new lineages of the Candidate Phyla Radiation and the Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota,Nanohaloarchaea superphyla and new lineages of the Planctomycete phylum with metabolic potential for both bio-remediation of the contamination as well as production of potentially hazardous secondary metabolites.We collected individual samples from each of four sites , the contamination source water as well as three wells. The domestic well water sample was clear and colorless in appearance with no odor. This water is pumped from ~100 m depth and used for human and cow consumption. Cow waste is pumped into the effluent lagoon , which was cloudy and brown in appearance with an apparent odor of ammonia and feces. After settlement of particulates, the lagoon water is used as a fertilizer source for the surrounding corn fields. Monitoring well 5 and monitoring well 6 are located immediately down gradient and upgradient respectively from a corn field receiving lagoon water . These wells are screened from 3 m to 10 m below ground surface . Monitoring well samples were clear and yellowish-green in appearance with a slight organic odor. Depth to the water table for the monitoring wells was 3.4 m bgs, and the wells were sampled at a depth of 4.3 m bgs. A previous hydrologic analysis indicated that MW5 is primarily recharged from the manured corn field, MW6 receives partial recharge from the manured corn field and partial recharge from an adjacent unmanured orchard, and DOM is primarily recharged from the adjacent orchard with slight impact from the manured field.Each water sample was tested for the presence of USDA pathogenic bacteria by inoculating liquid enrichment media and plating on selective nutrient media. The specific pathogens tested for were Salmonella, Enterococcus, Escherichia coli, and E. coli O157. Of these, Enterococcus, Escherichia coli, and E. coli O157 were detected in the LAG sample, but no pathogens were detected in any of the groundwater samples. Previous samplings from these and other similar monitoring wells on dairies did reveal the presence of USDA pathogens. However, it is not known how long these pathogens remain viable in the groundwater, and lagoon water had not recently been applied to the field where the monitoring wells are located. Our failure to detect these pathogens in groundwater suggests that they have a limited residence time.We asked whether the microbial composition of the water samples matched the chemical and culture-based observations. We analyzed the water microbial communities for DOM, LAG, MW5, and MW6 by constructing a whole metagenome library for each water sample and shotgun sequencing to a depth of ~50 million paired end 101 bp reads. We analyzed taxonomic makeup of the samples both by 16S rRNA gene profiling and whole metagenome assembly. First, we used EMIRGE to do reference-guided assembly of 16S ribosomal subunit genes and abundance estimation for each of our shotgun sequencing libraries.

We then assigned taxonomy to the 16S assemblies using the RDP web interface. Second, we assembled all of our reads and binned genomes from the assembled contigs and then assigned taxonomy to the genomic bins using RAPSEARCH to the UniProt UniRef100 database. The two metagenomic approaches we took are in good agreement with each other and with the water chemistry, however, we did not detect any of the cultured pathogens from the surface water by sequencing, suggesting they are rare. The shallow groundwater communities of MW5 and MW6 have similar species composition and are similar to the activated sludge bioreactor communities recently reported by Speth et al , a community sampled from the nitrogen removal stage of sewage wastewater treatment. However, in addition to observing 10 of the 12 phylogenetic groups reported by Speth, we additionally see 13 more in the groundwater. Specifically enriched are prokaryotes from the recently described nano-bacterial Parcubacteria and Microgenomates , nano-archaeal DPANN and ThaumarchaeotaAigarchaeota-Crenarchaeota-Korarchaeota superphyla as well as two distinct clades of Planctomycetes: the OM190 group, and the anammox Brocadiaceae group. Examining the EMIRGE data at abundances over 5%, the Archaea dominate, with the Crenarchaeote, Thermocladium , Woesearchaeota , and Methanomassiliicoccus . The Bacteria include anammox Planctomycetes from the Brocadia group , Acanthopleuribacter , Microgenomates genera , Dehalogenimonas , Parcubacteria genera , and Opitutus . The other major lineages in these samples include many known as nitrifiers, denitrifiers and methylotrophs as well as the heterotrophic eukaryote, Chlorella , at 7.4% and 5.4% respectively. In contrast to the similarities seen between the two shallow groundwater samples, the DOM and LAG samples each have their own distinct communities. The DOM sample is dominated by Domibacillusfollowed by Sphingomonasand Nitrospira . Anammox genomes are more rare in the deep groundwater, matching the trend seen in nitrate concentrations. The surface water is dominated by Rikenella ,which is known from animal feces. Several other likely animal-associated genera are abundant, including Anaerorhabdusand Acholeplasma , as well as a photosynthetic bacterium, Halochromatium . Overall, the taxonomic representation in the water samples matches well with expectations based on the chemical data. We note that several taxa appear unexpectedly in both the DOM and LAG samples, and we suspect these are contaminants in DOM from airborne dust. Specifically, Rikenella, the most dominant member of LAG, is present at 2.9% abundance in DOM. Likewise Anaerorhabdus, Coprobacillus, Halochromatium, and Acholeplasma are abundant at >5% in LAG and ~1% in DOM.

The proposal to place an upper limit on direct income payments was completely defeated

This preference was particularly important for the Eastern member states as they had less efficient bureaucracies and more generally struggled to find the administrative capacity necessary to implement the CAP. The Eastern Europe bloc therefore focused most of its political capital on pushing for a system that would redistribute direct payments while opposing a hard upper limit on those payments . The issue of limits on direct income payments was particularly contentious. This time, the new member states were involved, offering potential allies to both sides of the battle. The agricultural ministers of the Czech Republic, Romania, and Slovakia joined Germany, Italy, and the UK in signing a position paper that pledged to reject any agreement that included a cap on direct income payments . The paper argued that a cap on income payments contradicted CAP rules by discriminating against a set of farmers, in this case large farmers. It further argued that this policy would lead farms to split up or fail to merge. Preventing merges and compelling farms to split apart would reduce efficiency and result in more money being spent “administering the collateral costs of capping” income payments rather than supporting agriculture and advancing environmental goals . Though they were not signatories to the position paper, the Netherlands and Sweden also opposed capping. There were also new member states on the other side of the table. The main member states in favor of capping were Bulgaria, Poland, Austria, and the Baltic states. Bulgaria predicted that, unless capping was implemented, 4% of its farmers would receive 80% of the country’s direct payments. The situation in Poland was not as skewed,30l plant pots but there were still some farmers who received far more than others. Poland supported the plan because it viewed it as a way through which the vast disparities in payment levels between the member states could be addressed.

The Baltic states received among the lowest amount of direct payment per hectare, and thus also supported a payment limit. As very few Austrian farmers would be affected by capping, it was not costly for Austria to support the initiative. Finally, the French tacitly supported a payment cap , but did not wish to expend political capital on this soft preference against the far more vociferous “contra capping coalition” . One point of unity among all key actors was opposition to greening proposals36. COPACOGECA37 , the EU-wide farmer lobby, was particularly critical of the greening component that required farmers to set aside 7% of their land for ecological purposes. COPACOGECA warned that “it would imperil food security, require farmers to find ways of increasing production on remaining land, and damage the ability of farmers to respond to market signals”. Within the Council of Ministers, the general consensus was that the proposals were complex enough to lead to more red tape and bureaucracy and yet insufficient to actually meet the major environmental challenges at hand. The member states as a whole also expressed concern to the Commission that the proposals were too rigid and needed to be made more flexible, so they could be adapted to the circumstances and needs of each member state . Some also argued that the proportion of Pillar I committed to greening was too high at 30%. Here though, there was less consensus, and some thought that environmental matters should be left to Pillar 2, which concerns rural development, while Pillar 1 retained its focus on production and incomes. With respect to greening, the member states shared three goals or preferences: to increase flexibility in order to allow member states to tailor the policies to their own circumstances; to minimize the extent to which the measures would increase bureaucratic and administrative requirements; and to ensure that the greening measures not conflict with or impede production objectives . A first hurdle to tackle in reaching a final agreement was the greening proposals, all of which were widely condemned by the member states.

Beyond their overall negative reactions to the greening proposals, member states were concerned about how to make these policies work in their particular national contexts. As a result, the Commission relented and gave extensive concessions and exemptions in order to forge an agreement. The mandatory Ecological Focus Area was reduced from 7% to 5% of land. This reduction was made even though it was generally believed that most farmers already met EFA standards for 3.5% of their land. Thus they would only need to bring another 1.5% of their land into compliance to meet the new requirement. In addition, changes to rules on compliance and exceptions for certain groups of farmers resulted in 48% of arable land and 88% of arable goods farmers being exempted from the EFA requirement . For those roughly 12% of farmers who would be subjected to the EFA requirement, there was a long list of possible ways to meet this requirement38, some of which permitted the continued use of land and even fertilizers, essentially entirely undermining the idea behind requiring farmers to maintain ecological focus areas in the first place. Similar exemptions were achieved for permanent grassland measures. Concerning crop diversity, the initial proposal stipulated that farmers with more than 3 hectares of arable crops would be required to maintain three or more different crops simultaneously, with the largest crop not to exceed 70% of land and the smallest to represent at least 5% of the land. The proposal was revised, such that the rules applied only to farms over 10 hectares instead of 3 hectares. These farms are required to grow two crops, while those over 30 hectares must grow three, with the main crop not exceeding 75% of land . While this change to the minimum threshold for the holding size may seem like a minor change, it actually served to drastically reduce the number of farmers who were required to comply with this policy. As of 2013, two-thirds of farms in the EU consisted of 5 or less hectares of eligible land and one-fifth of member states reported an average holding size of under 10 hectares . Another point of contention in the proposal was the method and process for achieving both internal and external convergence. The Western member states were amenable to external convergence, as they admitted that an unequal CAP would be politically unsustainable in the long term. Despite this admission, there remained divisions between the old and new member states about how convergence should take place.

The new Eastern and Central European member states preferred rapid convergence while the older and predominately Western European member states preferred that the transition take place gradually. A compromise was reached between the old and new member states with the former accepting eventual convergence and the latter a gradual transition period that would not be complete until after 2020, projected to be in 2028. The Commission was able to forge an agreement, with both camps agreeing to some changes in how the policy would operate. While the new member states agreed to less redistribution at a slower rate than originally proposed, the older member states agreed to transition away from the payment schemes,30 litre pots available only to older member states, that were the source of the growing inequality. In addition, the older member states were afforded “hardship payments” to help farmers adjust to the changes in their income. Under the design of the external convergence program, only €738 million out of a total direct payment budget of €42.8 billion would be redistributed over the period 2014-2020 . As a point of comparison, €4.5 billion would have been needed if the plan had been to bring current payment levels up to parity immediately. The overall period of adjustment for external convergence would be gradual, beginning in 2014. Initially, farmers who previously received their payments based on a historical calculation would receive 50% of the new payment under that calculation while the other 50% would come via the new fixed payment per hectare system . Member states will have some discretion in how this shift takes place, with the expectation that the shift be gradual, and the requirement that by 2019, 100% of the payment be awarded according to the new model . Due to broad member state concerns about the effects of internal convergence, the Commission again relented, resulting in a process that would be slower and less abrupt for farmers. Under this compromise, the target for internal convergence was set at 60% for the “minimum level of average regional payment given to the individual beneficiary” . In addition, in a concession to France, member states were permitted to top up these payments in a sort of reverse degressivity. This position was defended on the grounds that, if production levels were to be maintained, as was the intention, then income supports should not be redirected from more productive to less productive farmers. Capping, in the form of a hard upper limit, once again went nowhere.

The central problem was that the Commission was the only major actor in favor of the proposal. Among the member states, there was a coalition that was staunchly opposed, with the rest of the member states not having a stake in the matter and thus deciding to stay out of the conflict rather than antagonize other member states for whom the issue mattered a great deal. In order to move the negotiations forward, the Commission completely dropped its proposal to place a cap on direct income payments. Degressivity, the other of the Commission’s main proposals seeking to manage payment thresholds, was included in the final agreement by way of a strategic concession by the Commission. Specifically, a final agreement on the issue of degressivity was facilitated by Germany’s acceptance of degressivity of 5% on all payments over €150,000. In exchange for this concession, the Commission allowed member states an additional way to meet the degressivity requirement. Member states were permitted to choose between 5% degressivity for all payments above €150,000, after employee and related costs were subtracted, or to apply a redistributive payment, accounting for at least 5% of the national envelope . Essentially, instead of subjecting individual farmers who earned over €150,000 to this 5% reduction, member states could choose to take 5% of their total national allotment of direct payments and redistribute it amongst their farming community. The final agreement ultimately watered down every element of the Commission’s proposal. For some proposals like external and internal convergence, the process was delayed. Others, like greening, involved the lowering of standards or weakening of requirements. Still others, like a cap on total payments, were defeated outright. The following table presents the initial proposal and final agreement side by side, and summarizes the main points of difference.Of the three central issue areas, nothing from the Commission’s initial proposal remained intact. The greening initiatives and the plan for achieving external convergence were both heavily revised.Regarding direct income payments, while the proposal to transition member states to a single formula for calculating direct payments survived, the plan for addressing the payment disparity between old and new member states was implemented more gradually, with a likely completion date of 2029 instead of 2020. The amount of degressivity was reduced to 5% for any farmer earning over €150,000 instead of a graduated system that would have seen up to 70% garnishment of payments for the largest earners. The overall package of reform for the direct payment system is the only element of the reform that had a clear link to disruptive politics: enlargement. The disruptive politics of enlargement created an opening for the Commission to implement actual change in how this system operated. As with past reforms, lessons from welfare state retrenchment can help explain this outcome. Specifically, the reform of this system serves as a good example of engaging in “vice into virtue” style retrenchment. Reformers were able to achieve policy retrenchment, particularly in area of payments to the largest, richest farmers, by working to correct an existing program that was functioning both unequally. Rather than attempting to adopt an entirely new program that directly attacked the most generous of direct payments, reformers instead focused on fixing an existing program. As a result, policymakers were able to achieve some retrenchment in the realm of direct payments.

Reactions in the member states to the appearance of Mad Cow were varied but swift

These challenges created an opportunity for Fischer to propose and advocate major reforms. They also informed the content of the proposed reforms. WTO pressures facilitated the consideration of policies that made the CAP less trade distorting. Enlargement allowed for deliberations over policies that would improve the financial sustainability of the CAP in the expanding Union. Concerns related to food safety and perceived public dissatisfaction allowed policies to be considered that increased environmental and animal welfare standards and that improved the equity of existing programs. Though these pressures were critical, the MTR was not launched in response to them. Rather, a provision requiring an MTR had been included in Agenda 2000 at Fischler’s insistence. Fischler had been frustrated by the tepid Agenda 2000 agreement and wanted another chance to enact meaningful reform. The MTR was a concession to Fischler in return for Chirac’s last minute revisions to Agenda 2000 at the 1999 Berlin Summit27. The purpose of the MTR was to assess the status of the implementation of Agenda 2000 and to offer improvements, if necessary . While Fischler hoped that the MTR would be his avenue for more far-reaching reform, he was careful to only refer to it as a review, and not a reform . Most member states assumed that the MTR would be merely a review and that no substantial changes would result. Still, pressure was building to overhaul the CAP, starting with the recently launched Doha Development Round. Fischler wanted to avoid a repeat of the Uruguay Round of GATT negotiations when stalemates in the agricultural sector had caused negotiations to drag out four years longer than planned. European manufacturing and services were angry at agriculture for the difficulties they faced in their negotiations because the structure of the CAP was a major obstacle to reaching a final agreement. Specifically, the EU,square plant containers with its trade-distorting price supports and production-based payments, was at the center of the controversy over how to structure the agricultural component of the GATT.

The European Commission and several member states concluded that entering the WTO negotiations with the CAP in violation of WTO rules once again would weaken the chances that EU representatives would be able to both defend the European Union’s vision for agricultural policy and also extract competitive agreements for services and manufacturing. In the run up to the Doha Round, European manufacturing and services sectors made it clear that they would not allow their bargaining position to be weakened or their interests threatened by European agriculture. These sectors signaled their intention to push aggressively for open markets “regardless of the price paid in terms of additional access to the EU agricultural market, which would presumably have to be borne by their fellow farmers” . In order to gain access to new markets, representatives for manufacturing and services stated their willingness to trade away core components of CAP policy. In addition, the European Commissioners for Trade, Competition, and Industry routinely challenged agriculture’s share of the EU budget and sought to diminish the prominence of the agriculture portfolio. Struggles and delays at the Doha Round caused by agriculture would provide additional ammunition to their efforts to siphon money from agriculture and into their own budgets. Given the clear signals sent by European manufacturing and services, Fischler wanted to enter the Doha Round with the ability to pursue and defend European agricultural interests without the sector being a stumbling block for progress towards Europe’s goals in other domains. The most important and fundamental way to position European agriculture for negotiation success was to ensure that CAP subsidies complied with existing WTO regulations. The WTO used a “subsidy stoplight28” system, containing green, amber, and blue boxes, to evaluate and classify member country subsidies. Permitted subsidies, meaning those that do not distort trade and do not include price supports, are in the green box. Examples of green box programs include decoupled subsidies and rural development supports. The amber box refers to all domestic subsidies that distort production and/or trade. Examples of amber box subsidies are production based subsidies and price supports. As subsidies in the amber box are considered trade and/or production distorting, they are subject to strict limitations, including an agreement to reduce them over time. In developed countries, only 5% of a country’s subsidies can fall into the amber box. Countries that exceed that limit must reduce their subsidies accordingly. The Uruguay Round agreement included a specific commitment by the 30 WTO members whose subsidies exceeded amber box limits to bring those subsidies in line with the 5% rule.

The last category is the blue box, which is also referred to as the “amber box with conditions”. It contains, “any support that would normally be in the amber box [which] also requires farmers to limit production” . It was developed as a way to help states move away from trade and production distorting amber box subsidies without causing too much hardship. Compliance in the agricultural sector was important because it meant that EU could press for market access for goods and services in emerging markets without being told that it first needed to get its agricultural policy in order. Keeping agriculture from hamstringing the pursuit of EU objectives for goods and services was also important for Fischler because it weakened the arguments often used by the EU Commissioners for Trade, Competition, and Industry to call for a reduction in the CAP budget. Speaking about his Doha Round strategy, Fischler noted, “we needed to change the conversation for the WTO. We couldn’t have a Uruguay Round repeat. We needed to be on the offensive. Decoupling was a good start because those types of subsidies [direct income support not linked to production] are already defined as in in the green box” . Fischler’s goal was to have the EU enter the round with its system of payments already in the “green box”. In order to do so, payments would have to be fully decoupled from production. Fischler felt that the EU would be better positioned in the negotiations if it came in with its agricultural subsidies already in compliance with WTO standards, rather than having to play catch up. Fischler was thus able to use the Doha Round to press the member states for more dramatic reform than they might have considered otherwise. Finally, Fischler knew that reform would be easier to pass if it occurred during an already scheduled CAP review, rather than in response to WTO negotiations . If reforms came during WTO negotiations, it would seem as though the CAP was caving into the demands of external actors. Fischler anticipated that the perception that non-EU actors were driving CAP reform would not play well with the public and would make reform even harder . Indeed, MacSharry’s approach to the 1992 reform was driven by this concern, as his reform overlapped with the GATT Uruguay Round. For this reason, MacSharry went to great lengths to present, explain, and justify his reform proposals as responses to internal EU needs and as improvements to CAP functionality rather than concessions to GATT officials, or, even worse, the United States.

Enlargement, like WTO negotiations, was placing pressure on the CAP. The accession of ten new member states,plastic pot manufacturers all poorer and less developed Central and East European countries, posed a serious threat to CAP spending. A CAP that remained coupled would be completely untenable under enlargement, when millions of new farmers would join the CAP. These new member states, where 26% of employment was in farming, were far more agrarian than the EU 15, which employed only 2-3% of the population in agriculture, and agriculture accounted for a much higher share of GDP . According to some estimates, the number of farmers in the EU would increase by 120% and the area of land under agricultural cultivation would increase by 42% after enlargement . The CAP would be responsible for providing income support to these farmers and rural development assistance for all agricultural land. Projections of the financial impact of enlargement suggested that, if the CAP remained unreformed, the budget would need to double. At the time of the MTR, the CAP was already the EU’s single largest program, consuming approximately 40% of the total EU budget. A doubling of CAP spending was political and financially unfeasible. At the time the member states agreed to the Agenda 2000 program, it was thought that the accession countries would not receive direct payments. The prevailing belief was that the new member states had no right to payments that were compensating current CAP farmers for price cuts they had been forced to accept in past reforms. The new member state farmers had not been part of the CAP at the time of these price cuts, and thus had no right to compensatory payments. In 2002, however, the Commission, anticipating that a two-tier CAP would be politically unsustainable, reversed course and decided that the accession states would be allowed to receive direct payments. The new member states would be allowed to access these payments gradually, not reaching payment levels commensurate to the existing EU-15 until 2013. Direct payments were to be phased in starting in 2004 from a base of 25% of the EU level upon accession and increasing by 5% per annum until 2007. Then, in 2008, the EU base payment would increase by 10% annually until 2013 at which point direct payments received by the new member states would be equal to the level of those received by the EU-15 . The graduated plan of access to the CAP bought EU reformers a small window of relief, but did not solve the fundamental problem of reconciling the existing CAP budget with the addition of ten new, largely agrarian, member states. Even though the new member states would not be integrated fully into the CAP payment system until 2013, Fischler needed to adopt change quickly because the new member states would be party to CAP negotiations upon formally joining the EU in 2004. Reform needed to happen before these new member states could enter and block changes that threatened to reduce the amount they received. The MTR was thus the last opportunity for reform before the new member states would be included in CAP negotiations. Enlargement’s threat to the budget gave Fischler a real, time-sensitive justification to push for massive and immediate changes in the operation of the CAP. These financial concerns allowed Fischler to construct a narrative that reform was not only desirable to improve the operation of the CAP, but necessary to save the CAP itself from total collapse. In addition, by arguing that decisions could be taken more easily now than after ten new member states joined the EU, he was able to make reform an immediate priority. Another problem facing the CAP was food safety. Consumer advocates were critical of the CAP in light of recent outbreaks of food-borne illnesses, and the apparent failure of the CAP to do anything to address or control them. Agenda 2000, the most recent CAP reform, did nothing to assuage these concerns, and on top of that inaction, new crises continued to emerge. In 1999, there was a dioxine crisis in Belgium and in 2001 an outbreak of foot and mouth disease in the UK . The UK’s second bovine spongiform encephalopathy crisis broke out in 1998 , concurrent with Agenda 2000 reform discussions. Logically, the BSE crisis in the UK should have compelled reformers to realize that existing environmental, animal welfare, and food safety standards were not sufficient. Making matters worse, new cases of BSE were detected in Belgium, France, Germany, Italy, and Spain. These crises also coincided with increasingly heated debates over the presence of GMOs in food for both human and livestock consumption .All of the member states with confirmed cases of BSE moved to quarantine areas near the infection and culled herds containing cows that tested positive. In France, officials also tripled funding for the study of BSE, ordered a review of slaughterhouse practices, banned the use of animal feed containing meat, and extended an import ban on British beef for a further three years beyond the EU-imposed ban.

There were three broad camps that emerged after the reform was officially announced

The income support system linked to production compounded the effect of these high prices, raising expenditure even further by fostering excess production that the EU was responsible for storing and dumping. Instead of proposing to scrap and redesign the entire program, an initiative almost certain to fail, MacSharry sought to make fundamental alterations to the aspects of the program that were most problematic. Specifically, MacSharry proposed lowering prices and removing land from production in order to address problems related to over production, ballooning costs, and environmental damage. The contents and overall design of the reform as elucidated in the leaked report were a product of MacSharry’s fundamental belief that price cuts and quota reductions were unavoidable. From his experience working on budgets in Ireland , MacSharry knew that “the EU could not keep spending 75% of its budget on agriculture. With twenty other Commissioners, one could not control 75% of spending” . Indeed, in Agricultural Council meetings, MacSharry warned that without correction, the CAP budget would overrun the ceiling placed upon agricultural spending. At the same time, MacSharry wanted to keep European farmers on the land. He argued that, “desertification of the rural area would be a disaster, and very expensive [for national governments]. There were no jobs, so people would be on the dole. The infrastructure already existed in the countryside for them: houses, roads, schools, and so on. If there was a rural exodus, new housing, roads, schools,blueberry production and so forth would have to be built to accommodate all of these people” . MacSharry’s plan for reform was first formally presented to the member states via a “Reflections Paper” published by the Commission in February of 1991.

The paper was purely qualitative and provided no specific numbers on cuts, compensation amounts, or any other action, unlike what had been published in the leaked document. The purpose of the document was to explain the proposed reforms in broad terms and to push forward and focus the debate on these matters . The Reflections Paper began with a discussion of two major problems that needed to be addressed in the subsequent reform. The first problem was production. Production had been increasing far more rapidly than consumption, resulting in the accumulation of massive stocks. Excess goods were then dumped onto an already stagnant world market, angering the EU’s trading partners. This problem of surplus dumping was one of the core sources of tension in the GATT that was forestalling progress toward an agreement. The second problem related to the economic well-being of farmers. Inequality was increasing. CAP support payments concentrated on the largest and most productive farms such that 20% of the Union’s farmers received 80% of the support. Meanwhile, the overall agricultural population continued to decline and the per capita income of farmers improved very little. This income problem highlighted a failure of the CAP to achieve one of the basic goals laid out in the Treaty of Rome, which formally proposed the creation of the CAP: to improve the standard of living and decrease income inequality in agriculture. These income problems persisted even though spending on the CAP was increasing rapidly from year to year The Commission argued that any reform undertaken needed to keep a sufficient number of farmers on the land because farmers play an essential role in preserving the natural environment and traditional landscapes of Europe. The Commission suggested that more emphasis be placed on this environmental role of the farmer and that rural development be promoted more broadly. Keeping farmers on the land was also tied to preserving the family farming model, which the Commission asserted was “the model…favoured by society generally” . Finally, the Commission suggested that reforms needed to make the agricultural budget “an instrument for real financial solidarity in favour of those in greatest need” by which it meant a more equitable distribution of support . Specifically, the Commission proposed that compensatory payments be modulated based on size of holding and income level. In other words, full compensation would be allowed up to a certain amount.

After that, the payment would be decreased, and the money saved would be redirected to smaller farmers and rural development objectives. Exemptions under quota and set-aside policies were also to be targeted towards the smallest and weakest producers. The broad objectives on market issues concerned re-balancing markets by bringing production back under control, promoting a system of production that relied on fewer inputs , and encouraging the spread of technology. The document singled out cereals, suggested that previous policies for the sector were flawed and instead proposed price cuts and mandatory removal of land from production. Lower cereals prices were needed to make European cereals competitive with animal feed substitutes, which were already a threat to grains in the European market, and stood to be an even bigger threat if and when the new GATT agreement was ratified. Overall, the contents of the Reflection Papers marked a sharp break with past practice in CAP reform . Instead of trying to make the existing system work, MacSharry was proposing systemic change and the, at least partial, introduction of new instruments, most notably a direct income payment. In addition, these reforms marked the first time that a proposal was made to directly and intentionally privilege smaller farmers. The proposals as first outlined by MacSharry in the leaked document were officially presented by the Commission in July of 1991, formally kicking off the CAP reform debate. This official proposal matched the specifics of the initial leaked document, with minor changes to the systems for managing price cuts and compensation payments. The Commission projected that, due to the extension of compensatory payments and the other new programs created for early retirement, afforestation, and environmental measures, total CAP spending would increase by 2.24 billion ECU. The Commission anticipated however, that expenditure on the CAP would decrease in the long run as stocks of cereals, beef, and dairy, as well as the agricultural population itself, declined . Still, estimates of how much savings there would be and how far into the future they would come were vague and unspecified.

The first group, Denmark, the Netherlands, and the United Kingdom, though in favor of the market liberalizing price cuts, was staunchly opposed to any “special treatment” for small farmers. Their farming sectors were comparatively large and efficient. With less protectionism and more liberalization, representatives for these member states believed that their farmers would gain market share at the expense of EU farmers who were propped up via protectionist policies. Countries in this group strongly opposed the Commission’s proposal to modulate aid in favor of small farmers, since their agricultural sectors were dominated by larger farmers. They believed that their farmers would be unfairly forced to bear the financial burden of sustaining unviable farms. In addition, the UK argued that modulated compensation offered farmers a perverse incentive to “split their holdings, becoming pensioners and non-competitive” . British Agricultural Minister John Gummer summed up his opposition to a program of modulation by stating that the United Kingdom was “not prepared to buy a reform at the expense of turning Europe’s agriculture into a tourist attraction for people who liked farming in Marie Antoinette style” . Fundamentally, all three countries advocated the largest possible price cuts with the lowest possible, ideally temporary,blackberry container size compensation administered at a flat rate to all farmers. France and Germany formed the second main group and maintained their traditional CAP alliance despite somewhat contradictory positions on the proposal. Germany, whose support for high prices, particularly for cereals, dated to the creation of the CAP , unsurprisingly objected to MacSharry’s proposed price cuts. German Agricultural Minister Ignaz Kiechle questioned the link between price cuts and production, suggesting that cutting prices for cereals would have little to no effect on production levels12 . That said, as one of the member states that underwrote most of the CAP budget, Germany generally favored all measures for controlling production, including set-asides and reductions of dairy quotas. France, however, rejected set-asides outright and was internally divided over the issue of price cuts for cereals. In addition to questioning the need for such a deep cut in cereal prices, French Minister Louise Mermaz called for the continuation of Community Preference, which would provide adequate protection for domestic supplies on the European market . Ireland’s main concern was over the cuts to beef prices, which it opposed even though it had no stake in the debate over cereals, as its cattle industry was grass fed. Finally, Ireland joined Germany and France in favor of a program of modulation that would focus support on the neediest farmers. This second group of countries was important for an additional reason.

Under the rules of Qualified Majority Voting France, Germany, and Ireland formed a blocking minority, while the Denmark, Netherlands, and the UK did not. So, while MacSharry and the Commission wanted to pass the reform with unanimous support, particular attention was directed to winning the cooperation of the group with the potential to block reform. All of the remaining countries, primarily the southern bloc, composed of Greece, Spain, Portugal, and Italy, accounted for the third group. The main agricultural sectors of these countries were largely excluded from the reform. Because farmers in these countries were typically poorer and less efficient than their northern counterparts, they stood to benefit from any redistributive programs, and thus were supportive of modulation. The minority of farmers in these countries that did grow products that were to be subjected to price cuts objected to a yield based calculation for compensation, as the yields in these countries were the lowest in the Union. If compensation for the price cuts was calculated on the basis of the historical yield in the area , then farmers in these countries would be paid a smaller direct income payment than farmers in other member states. Finally, Greece, Spain, and Portugal, and Italy most of all were particularly focused on the debates surrounding the milk quotas, hoping to preserve existing levels, if not increase them. The fact that every member state objected to at least some aspect of the program, and that many of these CAP positions put member states in direct opposition to one another, might seem to have doomed the negotiations from the start. However, as Dutch Farm Minister Piet Bukman, who chaired the Agricultural Council while the Netherlands held the rotating presidency noted, “all delegations agree that if there is no fundamental reform we will have an unbearable situation” . The common belief in the necessity of some kind of reform allowed for compromises to be made and an agreement to be reached, despite the wide range of positions held by the member states. In addition to internal conversations that each member state government held, part of the process of reaching the final agreement involved MacSharry and Arlindo Cunha, Portugal’s Agricultural Minister and Chairman of the Agricultural Council16 each working with the member states one by one. While Cunha toured the capitals in an effort to determine each country’s bottom line, MacSharry met with each minister privately, requesting a list of the 4-5 items most important to them. MacSharry agreed to include at least some of the requests in the reform in exchange for that minister’s support for the passage of the overall package of reforms . The bargaining that followed centered primarily on three core issues: level of price cuts for cereals, modulation, and milk quotas. Typically united in the CAP, France and Germany were divided over if and how much cereal prices should be cut. A major step in clearing the way for a deal was the reaching of an informal agreement between Mitterrand and Kohl. Germany agreed that it would not veto a cereal price cut in exchange for a promise from the French not to veto a new GATT deal, or at the bare minimum, to display “good will” . While Germany was opposed to any price cut for cereals since most of the farmers in the East grew that crop, it placed far more importance on a GATT deal. Conversely, France, favored cuts to cereal prices but had broad objections to liberalizing the CAP.